Semaphorin 4D/CD100 Antibody (133-1C6)

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Product Details

Summary
Reactivity Hu, Pm, Eq(-)Species Glossary
Applications ELISA, Flow, ICC/IF, ICC/IF
Clone
133-1C6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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Semaphorin 4D/CD100 Antibody (133-1C6) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47795)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Additional Information
Clone 133-1C6 was used by HLDA to establish CD designation.
Immunogen
PHA stimulated human peripheral blood lymphocytes. (Uniprot: Q92854)
Localization
Cell surface
Specificity
Recognizes a homodimeric protein comprised of 50kDa subunits, identified as CD100 (Workshop VI; Code N-L026). It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SEMA4D
Purity
Protein L or PEG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Use in ELISA reported in scientific literature (PMID: 29541402).
Optimal dilution for a specific application should be determined.
Publications
Read Publication using
NBP2-44362 in the following applications:

Reactivity Notes

Does not react with Equine.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein L or PEG purified

Alternate Names for Semaphorin 4D/CD100 Antibody (133-1C6)

  • A8
  • BB18
  • CD100 antigen
  • CD100
  • CD100M-sema-G
  • COLL-4
  • FLJ33485
  • FLJ34282
  • FLJ39737
  • FLJ46484
  • immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D
  • MGC169138
  • MGC169141
  • M-sema-G
  • sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D
  • SEMA4D
  • SEMAJ
  • Semaphorin 4D
  • semaphorin-4D

Background

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100, a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 4D/CD100 Antibody (NBP2-44362)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


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(1)
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SEMA4D
Uniprot