Semaphorin 4B Antibody - BSA Free

Western Blot: Semaphorin 4B Antibody [NBP2-19299] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299] - Mouse intestine. SEMA4B antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299] - U373 xenograft, using SEMA4B antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299] - Colon carcinoma. SEMA4B antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299] - Mouse intestine. SEMA4B antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Semaphorin 4B Antibody [NBP2-19299] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Semaphorin 4B antibody (NBP2-19299) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Semaphorin 4B Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Semaphorin 4B. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEMA4B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Semaphorin 4B Antibody - BSA Free

• KIAA1745sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4B
• MGC131831
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4B
• SEMA4B
• SEMAC
• Semaphorin 4B
• semaphorin-4B
• SemC

Background

Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SEMA4B