DCBLD2/ESDN Antibody

Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human/Mouse DCBLD2/ESDN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DCBLD2/ESDN at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

NCI‑H460 human large cell lung carcinoma cell line was stained with Human/Mouse DCBLD2/ESDN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6269, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

• Reactivity: Hu, Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

DCBLD2/ESDN Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DCBLD2/ESDN
  Gln67-Ala528
  Accession # Q96PD2
• Specificity: Detects human and mouse DCBLD2/ESDN in Western blots and human DCBLD2/ESDN in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DCBLD2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DCBLD2/ESDN Antibody

• CLCP1
• CLCP11700055P21Rik
• CUB, LCCL and coagulation factor V/VIII-homology domains protein 1
• DCBLD2
• discoidin, CUB and LCCL domain containing 2
• discoidin, CUB and LCCL domain-containing protein 2
• Endothelial and smooth muscle cell-derived neuropilin-like protein
• ESDN
• ESDNcoagulation factor V/VIII-homology domains protein 1

Background

DCBLD2 (discoidin, CUB, and LCCL domain containing 2), also known as ESDN (endothelial and smooth muscle cell derived neuropilin-like) and CLCP1, was identified through its up-regulation in a highly metastatic lung cancer cell line. DCBLD2 has structural similarities to neuropilins, VEGF receptors, and semaphorins, and is thought to play a role in cell motility and metastasis. SEMA4B is a receptor for DCBLD2/ESDN/CLCP1 and this interaction may be involved in regulating cancer cell metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DCBLD2/ESDN Antibody (AF6269)(1)

Publication using AF6269

• D Ali, D Alhattab, H Jafar, M Alzubide, N Sharar, S Bdour, A Awidi Differential Marker Expression between Keratinocyte Stem Cells and Their Progeny Generated from a Single Colony International Journal of Molecular Sciences, 2021-10-06;22(19):. 2021-10-06 [PMID: 34639148] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: DCBLD2
• Uniprot:
  • Q96PD2(Human)