Semaphorin 4C Antibody [Unconjugated]

Images

 
Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Semaphorin 4C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6125) at 15 µg/mL ...read more
Simple Western lane view shows lysates of SW13 human adrenal cortex adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Semaphorin 4C at approximately 121 kDa (as indicated) using 25 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Semaphorin 4C Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4C
Ala21-Gly663
Accession # Q9C0C4
Specificity
Detects human Semaphorin 4C in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse (rm) Sema4C is observed and less than 5% cross-reactivity with recombinant human (rh) Sema4G, rhSema4D, rhSema4B and rhSema4A is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SEMA4C
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 25 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF6125 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 4C Antibody [Unconjugated]

  • FLJ20369
  • KIAA1739
  • MGC126382
  • MGC126383
  • M-Sema F
  • M-SEMA-F
  • sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4C
  • SEMA4C
  • SEMACL1
  • Semaf
  • SEMAI
  • Semaphorin 4C
  • semaphorin-4C

Background

Sema4C (Semaphorin 4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature human Sema4C is a type I transmembrane glycoprotein that is 813 amino acids (aa) in length. It contains a 643 aa extracellular region (aa 21-663) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499‑551), and an Ig-like C2-type domain (aa 556-644). The cytoplasmic region interacts with PZD-domain containing proteins. There are three potential splice variants. One demonstrates an alternative start site at Met324, a second shows a deletion of aa 173-211, and a third contains an 89 aa substitution for aa 1-36. Over aa 21‑663, human Sema4C shares 85% aa identity with mouse Sema4C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Semaphorin 4C Antibody (AF6125)(2)

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Bioinformatics

Gene Symbol SEMA4C
Uniprot