Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Semaphorin 4C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6125) at 15 µg/mL ...read more
Simple Western lane view shows lysates of SW13 human adrenal cortex adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Semaphorin 4C at approximately 121 kDa (as indicated) using 25 µg/mL ...read more
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4C Ala21-Gly663 Accession # Q9C0C4
Detects human Semaphorin 4C in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse (rm) Sema4C is observed and less than 5% cross-reactivity with recombinant human (rh) Sema4G, rhSema4D, rhSema4B and rhSema4A is observed.
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Sema4C (Semaphorin 4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature human Sema4C is a type I transmembrane glycoprotein that is 813 amino acids (aa) in length. It contains a 643 aa extracellular region (aa 21-663) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499‑551), and an Ig-like C2-type domain (aa 556-644). The cytoplasmic region interacts with PZD-domain containing proteins. There are three potential splice variants. One demonstrates an alternative start site at Met324, a second shows a deletion of aa 173-211, and a third contains an 89 aa substitution for aa 1-36. Over aa 21‑663, human Sema4C shares 85% aa identity with mouse Sema4C.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.