Semaphorin 3F Antibody [Biotin]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Semaphorin 3F Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 3F
  Ala19-Pro775 (Arg583Ala & Arg586Ala)
  Accession # O88632
• Specificity: Detects human and mouse Semaphorin 3F in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Semaphorin 3D and rmSemaphorin 3E is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SEMA3F
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 3F Antibody [Biotin]

• sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
• sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F
• sema III/F
• sema IV
• Sema3F
• SEMA4
• SEMA-IV
• SEMAK
• Semaphorin 3F
• semaphorin III/F
• semaphorin IV

Background

Semaphorin 3F (Sema 3F; previously Sema IV) is one of six Class 3 (secreted) semaphorins which in the mouse share 40 - 50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon guidance and/or vascular tip cell guidance during development (1). Sema 3F is expressed in the developing nervous system, especially in the dorsal spinal cord (2, 3). In adults, Sema 3F is expressed in the lung and most other tissues (2). Crystal structures of semaphorins reveal that the 500 aa N-terminal Sema domain forms a seven-blade beta -propeller similar to that found in integrin molecules. Fourteen conserved cysteine residues and one or more N-glycosylation sites are thought to be critical for forming the secondary structure (4). Isoform A is missing aa 153 - 183 within the Sema domain relative to the long form (isoform B) but appears to have similar activity. C-terminal to the Sema domain, Sema 3F has a basic domain, a cysteine-knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, a cysteine for dimerization and another basic domain at the C-terminus. Dimerization and cleavage at the C-terminus are required for repulsing activity of class 3 semaphorins (5). Mouse Sema 3F shares 96%, 99%, 92%, 97% and 82% aa identity with human, rat, bovine, canine and chick Sema 3F, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors. Sema 3F signaling is transduced by type-A plexins, especially Plexin-A3, via interaction with neuropilin-2 (3, 6). Genetic disruption of either Sema 3F or neuropilin-2 alters motor axon trajectory to the ventral forelimb (3). Sema 3F is deleted or downregulated in many metastatic tumors. Restoration of Sema 3F decreases tumorigenicity, vascularization and adhesiveness, most likely through repulsive interactions, VEGF antagonism and downstream integrin regulation (7).

1. Kruger, R.P. et al. (2005) Nature Rev. Mol. Cell Biol. 6:789.
2. Eckhardt, F. and A. Meyerhans (1998) Neuroreport 9:3975.
3. Huber, A.B et al. (2005) Neuron 48:949.
4. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
5. Adams, R.H. et al. (1997) EMBO J. 16:6077.
6. Yaron, A. et al. (2005) Neuron 45:513.
7. Chedotal, A. et al. (2005) Cell Death Differ. 12:1044.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEMA3F
• Uniprot:
  • O88632()