Semaphorin 3B Antibody - BSA Free

Western Blot: Semaphorin 3B Antibody [NBP2-94522] - Analysis of extracts of various cell lines, using Semaphorin 3B at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Semaphorin 3B Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 569-748 of human SEMA3B (NP_001005914.1). SSRPALLEHKVFGVEGSSAFLECEPRSLQARVEWTFQRAGVTAHTQVLAEERTERTARGLLLRRLRRRDSGVYLCAAVEQGFTQPLRRLSLHVLSATQAERLARAEEAAPAAPPGPKLWYRDFLQLVEPGGGGSANSLRMCRPQPALQSLPLESRRKGRNRRTHAPEPRAERGPRSATHW
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEMA3B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Semaphorin 3B Antibody - BSA Free

• (semaphorin) 3B
• LUCA-1
• Sema A(V)
• sema domain, immunoglobulin domain (Ig), short basic domain, secreted
• Sema V
• SemA
• SEMA3B
• SEMA5
• SEMA5FLJ34863
• SEMAA
• Semaphorin 3B
• semaphorin A
• semaphorin V
• semaphorin-3B
• semaphorin-V
• SEMAV

Background

The semaphorin family of inhibitory axon guidance molecules includes secreted, transmembrane, and GPI anchored extracellular molecules. These proteins regulate axon guidance by inhibiting axons from growing toward incorrect targets. SEMA3B inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEMA3B