Secretagogin Antibody - BSA Free

Western Blot: Secretagogin Antibody [NBP3-05545] - Western blot analysis of tissue lysates using Secretagogin Antibody at a dilution of 1:1,000 in green: [1] protein standard, [2] mouse olfactory bulb [3] rat cerebellum, and [4] cow cerebellum. The band at ~27kDa corresponds to the secretagogin protein. Secretagogin protein is highly expressed in the mouse olfactory bulb, but significantly less in the cerebellum.

Immunocytochemistry/Immunofluorescence: Secretagogin Antibody [NBP3-05545] - Immunofluorescent analysis of mouse brain olfactory bulb section stained with Secretagogin Antibody at a dilution of 1:1,000, in red, and costained with mouse mAb to calretinin at a dilution of 1:500 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. Secretagogin is highly expressed in the mitral and granular cell layers, while calretinin is in the glomerular layer. Cells that express Secretagogin or calretinin only are red or green respectively. A number of cells containing both proteins appears orange-yellow.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Secretagogin Antibody - BSA Free Summary

• Description: Novus Biologicals Chicken Secretagogin Antibody - BSA Free (NBP3-05545) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length recombinant human Secretagogin protein
• Clonality: Polyclonal
• Host: Chicken
• Gene: SCGN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:1000-1:2000
  • Western Blot 1:1000-5000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol.
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Secretagogin Antibody - BSA Free

• CALBL
• DJ501N12.8
• SCGN
• SECRET
• Secretagogin
• secretagogin, EF-hand calcium binding protein
• SECRETsecretagogin
• SEGN
• Setagin

Background

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCGN