SEC61A Antibody

Images

 
Western Blot: SEC61A Antibody [NBP1-31267] - Whole cell extracts and extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC61A1 antibody at a dilution of 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: SEC61A Antibody [NBP1-31267] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SEC61A Antibody [NBP1-31267] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, RM, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SEC61A Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SEC61A1. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Zebrafish (100%), Rhesus Macaque (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEC61A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SEC61A Antibody

  • HSEC61
  • protein transport protein SEC61 alpha subunit
  • protein transport protein Sec61 subunit alpha isoform 1
  • protein transport protein Sec61 subunit alpha
  • Sec61 alpha 1 subunit (S. cerevisiae)
  • Sec61 alpha-1
  • sec61 homolog
  • SEC61
  • SEC61A

Background

SEC61A belongs to the SECY/SEC61 alpha family. It has similarity to a mouse protein which suggests a role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum.The SEC61 complex is responsible for translocation of proteins across the rough endoplasmic reticulum membrane. SEC61 is composed of three subunits: alpha, beta, and gamma; that associate to form a heterotrimer. Multiple heterotrimers associate forming the ER translocon. SEC61 alpha is a polytopic protein that has ten membrane-spanning loops. SEC61 has been found to be tightly associated with membrane bound ribosomes, either directly or through adapter proteins, and is required for assembly of membrane and secretory proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SEC61A1