SDHB Antibody (7D12) - BSA Free

Immunohistochemistry: SDHB Antibody (7D12) [NBP3-26332] - Image of SDHB Antibody (7D12) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry: SDHB Antibody (7D12) [NBP3-26332] - Image of SDHB Antibody (7D12) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: SDHB Antibody (7D12) [NBP3-26332] - Positive Western Blot detected in: 293 whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate, PC-3 whole cell lysate, Rat heart tissue, Mouse heart tissue, Mouse liver tissue. All lanes: SDHB Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa Observed band size: 32 kDa

Flow Cytometry: SDHB Antibody (7D12) [NBP3-26332] - Overlay histogram showing 293 cells stained with SDHB Antibody (7D12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, IHC
• Clone: 7D12
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SDHB Antibody (7D12) - BSA Free Summary

• Description: Novus Biologicals Rabbit SDHB Antibody (7D12) - BSA Free (NBP3-26332) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human SDHB [UniProt P21912]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SDHB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:20-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SDHB Antibody (7D12) - BSA Free

• EC 1.3.5.1
• FLJ92337
• IP
• Iron-sulfur subunit of complex II
• PGL4
• SDH
• SDH1
• SDH2
• SDHIP
• succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
• succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Background

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SDHB