SCF/c-kit Ligand Antibody [Biotin]

Images

 
There are currently no images for SCF/c-kit Ligand Antibody (BAF455).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations

SCF/c-kit Ligand Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant mouse SCF (R&D Systems, Catalog # 455-MC)
Lys26-Ala189
Accession # Q78ED8
Specificity
Detects mouse SCF in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant human SCF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Kitl
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Mouse SCF/c-kit Ligand Antibody (Catalog # AF-455-NA)
ELISA Detection: Mouse SCF/c-kit Ligand Biotinylated Antibody (Catalog # BAF455)
Standard: Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC)
Readout System
Publications
Read Publications using
BAF455 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCF/c-kit Ligand Antibody [Biotin]

  • c-kit Ligand
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • KIT ligand
  • Kitl
  • KITLG
  • KL-1
  • Mast cell growth factor
  • MGF
  • MGFSHEP7
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SLF
  • steel factor

Background

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 - 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72% - 75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).

  1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
  2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
  3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
  4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
  5. Kapur, R. et al. (2002) Blood 100:1287.
  6. Wang, C-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
  8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327. 
  9. Huang, E. et al. (1990) Cell 63:225.
  10. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
  11. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
  12. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
  13. Flanagan, J.G. et al. (1991) Cell 64:1025.
  14. Martin, F.H. et al. (1990) Cell 63:203.
  15. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
  16. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1356
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-13967
Species: Hu
Applications: IHC, IHC-P
NBP1-80670
Species: Hu
Applications: IHC, IHC-P
BAF455
Species: Mu
Applications: WB, ELISA(Det)

Publications for SCF/c-kit Ligand Antibody (BAF455)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow, Functional Assay, Luminex Assay Development, Neut.


Filter By Application
Flow
(1)
Functional Assay
(1)
Luminex Assay Development
(1)
Neut
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(3)
All Species

Reviews for SCF/c-kit Ligand Antibody (BAF455) (0)

There are no reviews for SCF/c-kit Ligand Antibody (BAF455). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SCF/c-kit Ligand Antibody (BAF455) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for SCF/c-kit Ligand Antibody (BAF455)

Discover related pathways, diseases and genes to SCF/c-kit Ligand Antibody (BAF455). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SCF/c-kit Ligand Antibody (BAF455)

Discover more about diseases related to SCF/c-kit Ligand Antibody (BAF455).
 

Pathways for SCF/c-kit Ligand Antibody (BAF455)

View related products by pathway.

PTMs for SCF/c-kit Ligand Antibody (BAF455)

Learn more about PTMs related to SCF/c-kit Ligand Antibody (BAF455).

Blogs on SCF/c-kit Ligand

There are no specific blogs for SCF/c-kit Ligand, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SCF/c-kit Ligand Antibody [Biotin] and receive a gift card or discount.

Bioinformatics

Gene Symbol Kitl
Entrez
Uniprot