SCF/c-kit Ligand Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

SCF/c-kit Ligand Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse SCF (R&D Systems, Catalog # 455-MC)
  Lys26-Ala189
  Accession # Q78ED8
• Specificity: Detects mouse SCF in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant human SCF is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Kitl
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse SCF/c-kit Ligand Antibody (Catalog # AF-455-NA)
  ELISA Detection: Mouse SCF/c-kit Ligand Biotinylated Antibody (Catalog # BAF455)
  Standard: Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCF/c-kit Ligand Antibody [Biotin]

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 - 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72% - 75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).

1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
5. Kapur, R. et al. (2002) Blood 100:1287.
6. Wang, C-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327. 
9. Huang, E. et al. (1990) Cell 63:225.
10. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
11. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
12. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
13. Flanagan, J.G. et al. (1991) Cell 64:1025.
14. Martin, F.H. et al. (1990) Cell 63:203.
15. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
16. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SCF/c-kit Ligand Antibody (BAF455)(5)

Publications using BAF455

• Rai , Shinya, Tanaka , Hirokazu, Suzuki , Mai, Ogoh , Honami, Taniguchi , Yasuhiro, Morita , Yasuyosh, Shimada , Takahiro, Tanimura , Akira, Matsui , Keiko, Yokota , Takafumi, Oritani , Kenji, Tanabe , Kenji, Watanabe , Toshio, Kanakura , Yuzuru, Matsumura , Itaru Clathrin assembly protein CALM plays a critical role in KIT signaling by regulating its cellular transport from early to late endosomes in hematopoietic cells. PLoS ONE, 2014-10-03;9(10):e109441. 2014-10-03 [PMID: 25279552] (Neutralization, Mouse)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Dolgachev VA, Ullenbruch MR, Lukacs NW, Phan SH Role of stem cell factor and bone marrow-derived fibroblasts in airway remodeling. Am. J. Pathol., 2009-01-15;174(2):390-400. 2009-01-15 [PMID: 19147822] (Functional Assay, Mouse)
• Higuchi K, Ayach B, Sato T, Chen M, Devine SP, Rasaiah VI, Dawood F, Yanagisawa T, Tei C, Takenaka T, Liu PP, Medin JA Direct injection of kit ligand-2 lentivirus improves cardiac repair and rescues mice post-myocardial infarction. Mol. Ther., 2008-11-11;17(2):262-8. 2008-11-11 [PMID: 19002160] (Flow Cytometry, Western Blot, Human, Mouse)
• Yoshihiro Ueda, Motonari Kondo, Garnett Kelsoe Inflammation and the reciprocal production of granulocytes and lymphocytes in bone marrow The Journal of Experimental Medicine 2005-06-06 [PMID: 15939792] (ELISA Detection, Mouse)

Bioinformatics

• Gene Symbol: Kitl
• Uniprot:
  • Q78ED8()