SCF/c-kit Ligand Antibody [Unconjugated]

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Recombinant MouseSCF/c‑kit Ligand (Catalog #455‑MC) stimulates prolif­eration in the TF‑1 human erythro­leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

SCF/c-kit Ligand Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse SCF/c-kit Ligand
Lys26-Ala189
Accession # Q78ED8
Specificity
Detects mouse SCF/c-kit Ligand in ELISAs and Western blots. In Western blots, less than 20% cross-reactivity with recombinant human SCF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Kitl
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.3-0.6 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse SCF/c-kit Ligand Antibody (Catalog # AF-455-NA)
ELISA Detection: Mouse SCF/c-kit Ligand Biotinylated Antibody (Catalog # BAF455)
Standard: Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC)
Publications
Read Publications using
AF-455-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCF/c-kit Ligand Antibody [Unconjugated]

  • c-kit Ligand
  • DCUA
  • DFNA69
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • FPHH
  • KIT ligand
  • Kitl
  • KITLG
  • KL-1
  • Mast cell growth factor
  • MGF
  • MGFSHEP7
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SHEP7
  • SLF
  • steel factor

Background

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1-7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72-75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Non-covalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).

  1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
  2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
  3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
  4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
  5. Kapur, R. et al. (2002) Blood 100:1287.
  6. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
  8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327. 
  9. Huang, E. et al. (1990) Cell 63:225.
  10. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
  11. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
  12. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4671.
  13. Flanagan, J.G. et al. (1991) Cell 64:1025.
  14. Martin, F.H. et al. (1990) Cell 63:203.
  15. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
  16. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SCF/c-kit Ligand Antibody (AF-455-NA)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 7 applications: ELISA Development, ICC, IHC, IHC-Fr, Luminex Development, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
ICC
(1)
IHC
(1)
IHC-Fr
(1)
Luminex Development
(1)
Neutralization
(2)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF-455-NA Applications Species
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012;58(2):169-77. 2012 [PMID: 22277799] (Luminex Development, Mouse) Luminex Development Mouse
Gu Y, Runyan C, Shoemaker A, Surani A, Wylie C Steel factor controls primordial germ cell survival and motility from the time of their specification in the allantois, and provides a continuous niche throughout their migration. Development, 2009;136(8):1295-303. 2009 [PMID: 19279135] (IHC-Fr, Mouse) IHC-Fr Mouse
Dolgachev VA, Ullenbruch MR, Lukacs NW, Phan SH Role of stem cell factor and bone marrow-derived fibroblasts in airway remodeling. Am. J. Pathol., 2009;174(2):390-400. 2009 [PMID: 19147822] (ELISA Development, Mouse) ELISA Development Mouse
Huang B, Lei Z, Zhang GM, Li D, Song C, Li B, Liu Y, Yuan Y, Unkeless J, Xiong H, Feng ZH SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment. Blood, 2008;112(4):1269-79. 2008 [PMID: 18524989] (Western Blot, Mouse) Western Blot Mouse
Milenkovic N, Frahm C, Gassmann M, Griffel C, Erdmann B, Birchmeier C, Lewin GR, Garratt AN Nociceptive tuning by stem cell factor/c-Kit signaling. Neuron, 2007;56(5):893-906. 2007 [PMID: 18054864] (ICC, Mouse) ICC Mouse
Mak SS, Moriyama M, Nishioka E, Osawa M, Nishikawa S Indispensable role of Bcl2 in the development of the melanocyte stem cell. Dev. Biol., 2006;291(1):144-53. 2006 [PMID: 16427619] (IHC, Mouse) IHC Mouse
Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice. Arch. Dermatol. Res., 2001;293(10):508-14. 2001 [PMID: 11820727] (Neutralization, Mouse) Neutralization Mouse
Shih CC, Hu MC, Hu J A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells. Blood, 2000;95(6):1957-66. 2000 [PMID: 10706861] (Neutralization, Mouse) Neutralization Mouse

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