Scaffold attachment factor B2 Antibody (4H3) - Azide and BSA Free

Western Blot: Scaffold attachment factor B2 Antibody (4H3) [H00009667-M01] - Analysis of SAFB2 expression in transfected 293T cell line by SAFB2 monoclonal antibody (M01), clone 4H3.Lane 1: SAFB2 transfected lysate (Predicted MW: 57.2 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: Scaffold attachment factor B2 Antibody (4H3) [H00009667-M01] - Detection limit for recombinant GST tagged SAFB2 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 4H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Scaffold attachment factor B2 Antibody (4H3) - Azide and BSA Free Summary

• Immunogen: SAFB2 (NP_055464, 103 a.a. ~ 199 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NGLEDDSRDGQEDMEASLENLQNMGMMDMSVLDETEVANSSAPDFGEDGTDGLLDSFCDSKEYVAAQLRQLPAQPPEHAVDGEGFKNTLETSSLNFK
• Specificity: SAFB2 - scaffold attachment factor B2 (4H3)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SAFB2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Scaffold attachment factor B2 Antibody (4H3) - Azide and BSA Free

• KIAA0138SAF-B2
• scaffold attachment factor B2

Background

Scaffold attachment factor B2 (SAFB2) is a chromatin associated protein that interacts with DNA elements termed S/MARs (scaffold or matrix attachment regions) but is not part of the nuclear matrix. SAFB2 is structurally and functionally similar to SAFB1 and appears to be a multifunctional protein involved in chromatin organization, mRNA processing and transcriptional regulation. SAFB2 has been demonstrated to interact with and function as a corepressor with many nuclear receptors such as estrogen receptor-alpha, PPAR-gamma, and FXR-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAFB2
• Uniprot:
  • NP_055464(Human)