SATB2 Antibody (SATB2/4374R)

Immunohistochemistry-Paraffin: SATB2 Antibody (SATB2/4374R) [NBP3-07487] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with SATB2 Rabbit Recombinant Monoclonal Antibody (SATB2/4374R).

Immunohistochemistry-Paraffin: SATB2 Antibody (SATB2/4374R) [NBP3-07487] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with SATB2 Rabbit Recombinant Monoclonal Antibody (SATB2/4374R).

• Reactivity: Hu
• Applications: IHC
• Clone: SATB2/4374R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SATB2 Antibody (SATB2/4374R) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08808)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide corresponding to residues within aa200-300 corresponding to human SATB2 (Uniprot: Q9UPW6)
• Localization: Nuclear
• Marker: Colorectal Carcinoma Marker
• Specificity: SATB2 is a DNA binding protein that specifically binds nuclear matrix attachment regions. It is involved in transcription regulation and chromatin remodeling. SATB2 expression in colorectal carcinomas (CRC) is correlated with good prognosis and in laryngeal squamous cell carcinoma it functions as a tumor suppressor wherein loss of expression is positively correlated with high tumor grade and recurrence. Moreover, SATB2, in combination with CK20, could identify almost all CRC s. Upper gastrointestinal (GI) carcinomas and pancreatic ductal carcinomas are usually negative for SATB2, and ovarian carcinomas, lung adenocarcinomas, and adenocarcinomas from other origin are rarely positive for SATB2. Additionally, SATB2 antibody can identify neuroendocrine neoplasms of colon and rectum because SATB2 is usually negative in neuroendocrine neoplasms of the GI tract, pancreas, and lung. More recently, it has been reported that SATB2 is a sensitive marker for tumors with osteoblastic differentiation.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SATB2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for SATB2 Antibody (SATB2/4374R)

• DNA-binding protein SATB2
• FLJ32076
• KIAA1034FLJ21474
• MGC119474
• MGC119477
• SATB family member 2
• SATB homeobox 2
• Special AT-rich sequence-binding protein 2

Background

SATB2 binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SATB2
• Uniprot:
  • Q9UPW6(Human)