SATB2 Antibody (CL0320) - BSA Free

Immunohistochemistry-Paraffin: SATB2 Antibody (CL0320) [NBP2-52914] - nalysis in human rectum and liver tissues using NBP2-52914 antibody. Corresponding SATB2 RNA-seq data are presented for the same tissues.

Western Blot: SATB2 Antibody (CL0320) [NBP2-52914] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: SATB2 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Immunocytochemistry/Immunofluorescence: SATB2 Antibody (CL0320) [NBP2-52914] - Staining of U-138 MG cells using the Anti-SATB2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: SATB2 Antibody (CL0320) [NBP2-52914] - Staining of rat hippocampus shows no positivity in neurons in dentate gyrus as expected.

Western Blot: SATB2 Antibody (CL0320) [NBP2-52914] - Lane 1: Marker [kDa], Lane 2: Human cell line HELA

Immunohistochemistry-Paraffin: SATB2 Antibody (CL0320) [NBP2-52914] - Staining of human rectum shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: SATB2 Antibody (CL0320) [NBP2-52914] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: SATB2 Antibody (CL0320) [NBP2-52914] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: SATB2 Antibody (CL0320) [NBP2-52914] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: SATB2 Antibody (CL0320) [NBP2-52914] - Staining of rat hippocampus shows moderate to strong nuclear positivity in CA1 layer neurons.

• Reactivity: Hu, Rt, Mu
• Applications: WB, ICC/IF, IHC
• Clone: CL0320
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

SATB2 Antibody (CL0320) - BSA Free Summary

• Description: Novus Biologicals Mouse SATB2 Antibody (CL0320) - BSA Free (NBP2-52914) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q9UPW6, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SATB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
• Control Peptide:

  SATB2 Recombinant Protein Antigen (NBP2-52914PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for SATB2 Antibody (CL0320) - BSA Free

• DNA-binding protein SATB2
• FLJ32076
• KIAA1034FLJ21474
• MGC119474
• MGC119477
• SATB family member 2
• SATB homeobox 2
• Special AT-rich sequence-binding protein 2

Background

SATB2 binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SATB2