SATB1 Antibody

Images

 
Western Blot: SATB1 Antibody [NBP1-51960] - NBP1-51960 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: SATB1 Antibody [NBP1-51960] - (3.8ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

SATB1 Antibody Summary

Immunogen
Peptide with sequence C-ESDEENRQKTRPRT corresponding to internal region according to NP_002962.1.
Epitope
C-ESDEENRQKTRPRT
Specificity
Reported variants NP_001124482.1 and NP_002962.1 represent identical protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SATB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Experiments gave bands at approx. 110 kDa band and 85 kDa band in MOLT4 lysates after 0.3ug/ml antibody staining. The observed 110 kDa band corresponds to earlier findings in scientific literature with different antibodies (PMID: 9548713). This protein has a calculated MW of 86.0 kDa band according to NP_002962.1. IHC-P: Human thymus shows select nuclear staining.
Publications
Read Publication using NBP1-51960.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SATB1 Antibody

  • DNA-binding protein SATB1
  • SATB homeobox 1
  • SATB1
  • special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's)
  • Special AT-rich sequence-binding protein 1

Background

The protein binds to DNA at special AT-rich sequences at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SATB1 Antibody (NBP1-51960)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SATB1
Entrez
Uniprot