SATB1 Antibody

Western Blot: SATB1 Antibody [NBP1-51960] - NBP1-51960 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP1-51960] - (3.8ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SATB1 Antibody Summary

• Description: Novus Biologicals Goat SATB1 Antibody (NBP1-51960) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-SATB1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ESDEENRQKTRPRT corresponding to internal region according to NP_002962.1.
• Epitope: C-ESDEENRQKTRPRT
• Specificity: Reported variants NP_001124482.1 and NP_002962.1 represent identical protein.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SATB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Experiments gave bands at approx. 110 kDa band and 85 kDa band in MOLT4 lysates after 0.3ug/ml antibody staining. The observed 110 kDa band corresponds to earlier findings in scientific literature with different antibodies (PMID: 9548713). This protein has a calculated MW of 86.0 kDa band according to NP_002962.1. IHC-P: Human thymus shows select nuclear staining.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SATB1 Antibody

• DNA-binding protein SATB1
• SATB homeobox 1
• SATB1
• special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's)
• Special AT-rich sequence-binding protein 1

Background

The protein binds to DNA at special AT-rich sequences at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SATB1 Antibody (NBP1-51960)(1)

Publication using NBP1-51960

• Tan JA, Sun Y, Song J et al. SUMO conjugation to the matrix attachment region-binding protein, special AT-rich sequence-binding protein-1 (SATB1), targets SATB1 to promyelocytic nuclear bodies where it undergoes caspase cleavage. J Biol Chem 2008-06-01 [PMID: 18408014]

Bioinformatics

• Gene Symbol: SATB1
• Entrez:
  • 6304(Human)
  • 20230(Mouse)
  • 316164(Rat)
• Uniprot:
  • NP_002962.1(Human)