Human SAT1 ELISA Kit (Colorimetric) Summary
                         
                                
                                
                                
            | Description | Assay Length: 4.5 hours | 
            | Standard Curve Range | 0.781 - 50 ng/mL (example only; lot dependent) | 
            | Sensitivity | 0.29 ng/mL (example only; lot dependent) | 
            | Assay Type | Sandwich ELISA | 
            | Inter-Assay | %CV < 12 (example only; lot dependent) | 
            | Intra-Assay | %CV < 10 (example only; lot dependent) | 
            | Sample Volume | 100 uL | 
            | Kit Type | ELISA Kit (Colorimetric) | 
            | Gene | SAT1 | 
                                
                          Applications/Dilutions
                                 Packaging, Storage & Formulations
            | Storage | Storage of components varies.  See protocol for specific instructions. | 
Kit Components
                                
                                
                                        
                                                | Components | Detection Reagent ADetection Reagent BDiluent BufferInstruction manualPlate sealer for 96 wellsPre-coated 96T strip plateStandardStop SolutionTMB SubstrateWash Buffer (30 x concentrate)
 | 
                                
                         Alternate Names for Human SAT1 ELISA Kit (Colorimetric)
                     Background
 
                    
                    SAT1--also known as Spermidine/spermine-N1-acetyltransferase (SSAT1)--is a short-lived polyamine catabolic enzyme which catalyzes the acetylation of spermidine and spermine. SAT1 is a highly regulated enzyme which allows a fine attenuation of the intracellular concentration of polyamines.  Induction of SAT1 plays an important role in polyamine homoeostasis, since the N1-acetylated polyamines can be excreted or oxidized by acetylpolyamine oxidase.
                      Limitations
 
                    
                    This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are 
guaranteed for 6 months from date of receipt.
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                  Product General Protocols
                        
                        Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
                        
FAQs for SAT1 ELISA Kit (NBP3-40094). (Showing 1 - 2 of 2 FAQ).
                        
                        - 
                                    I need to radiolabel (iodinate) the SAT1 antibody. Which one should I use?
                                
                                    - I would recommend NB110-41622 for you as it is a well validated antibody with very clean QC validation data, multiple applications/broad species reactivity. Additionally, this antibody has been cited by Liao et al. in The Journal of Biological Chemistry 2009;284(12):8174-8184 (https://www.jbc.org/article/S0021-9258(20)32529-1/fulltext).
 
- 
                                    wondering what the difference is between your quantikine and duo set elisas?
                                
                                    - Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
 
   
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