SARM1 Antibody (754711) [Alexa Fluor™ Plus 680]

• Clone: 754711
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

SARM1 Antibody (754711) [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects human SARM1 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

SARM1 (Sterile alpha and TIR motif-containing protein 1; also SAM domain-containing protein 2 and TIR-1 homolog) is a 73-75 kDa member of the TLR adaptor family of molecules. It is both nuclear and cytoplasmic, and expressed in monocytes, neurons, and retinal photoreceptor cells, where it demonstrates disparate activities. In monocytes, SARM1 is a specific inhibitor of TRIF-dependent TLR-3 and -4 signaling, and appears to block MAPK phosphorylation. In neurons, SARM1 regulates microtubule stability, and thus axon and dendrite elongation. And in retinal photoreceptor cells, SARM1 complexes with Na/K ATPase to create a cell surface receptor for retinoschisin. Human SARM1 is 724 amino acids (aa) in length. It contains an N-terminal polybasic motif (aa 1-20) and a Gly-rich region (aa 22-90) that may anchor the molecule to intracellular membranes. It also possesses two SAM domains (aa 412-548) plus a TIR region (aa 559-657) that appears to interact with TIRF. Proteolytic cleavage products of 35 kDa and 30 kDa have been described. There is one splice variant that shows a 72 aa substitution for aa 1-106. Over aa 556-724, human SARM1 shares 94% aa identity with mouse SARM1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.