SAR1B Antibody

Images

 
Western Blot: SAR1B Antibody [NBP1-32725] - Adult mouse small intestine homogenate, 20 ug total protein per lane. Antibody at 1:1000. Secondary HRP conjugated antibody at 1:5000. Bands visualized with Immobilon Western ...read more
Immunocytochemistry/ Immunofluorescence: SAR1B Antibody [NBP1-32725] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725] - Mouse ovary. SAR1B antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: SAR1B Antibody [NBP1-32725] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE, antibody diluted at 1:3000.
Western Blot: SAR1B Antibody [NBP1-32725] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE, antibody diluted at 1:1000.
Western Blot: SAR1B Antibody [NBP1-32725] - A. 50 ug rat brain lysate/extract 12 % SDS-PAGE SAR1B antibody dilution: 1:3000
Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SAR1B Antibody Summary

Immunogen
Full length human SAR1B Recombinant protein.
Localization
Endoplasmic reticulum membrane, Peripheral membrane protein, Golgi apparatus, Golgi stack membrane, Peripheral membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SAR1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
SAR1B antibody validated for WB from a verified customer review.
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-32725 in the following applications:

Publications
Read Publication using
NBP1-32725 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for SAR1B Antibody

  • 2310075M17Rik
  • ANDD
  • CMRD
  • EC 3.6.5
  • GTP-binding protein B
  • GTP-binding protein SAR1b
  • GTP-binding protein Sara
  • SAR1 homolog B (S. cerevisiae)
  • SAR1a gene homolog (S. cerevisiae) 2
  • SAR1a gene homolog 2 (S. cerevisiae)
  • SAR1a gene homolog 2
  • SARA2GTBPB
  • SARB

Background

SAR1B belongs to the small GTPase superfamily, SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus and is activated by the guanine nucleotide exchange factor PREB. SAR1B is involved in the selection of the protein cargo and the assembly of the COPII coat complex. Defects in SAR1B are the cause of chylomicron retention disease (CMRD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The conditions are characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-20261
Species: Hu
Applications: WB, ICC/IF
H00008826-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA, KD
NBP1-82456
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
NB200-527
Species: Hu, Mu, Rt
Applications: WB, IP
NBP1-62489
Species: Hu, Mu, Dr
Applications: WB
NBP1-32870
Species: Hu, Ch
Applications: WB, IHC, IHC-P, IP, KD
NBP2-93525
Species: Hu, Rt
Applications: WB, ICC/IF
PP-H4417-00
Species: Hu
Applications: WB, IP, DirELISA
MAB90551
Species: Hu, Mu, Rt
Applications: WB
NBP2-34842
Species: Hu, Mu, Rt, Ha
Applications: WB, Flow, ICC/IF, IHC, IP
NBP2-02377
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-84957
Species: Hu
Applications: WB, IHC, IHC-P
H00001406-M02
Species: Hu, Mu, Bv, Fi
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-53420
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB600-609
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP1-86263
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-84412
Species: Hu
Applications: IHC, IHC-P
NBP1-32725
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SAR1B Antibody (NBP1-32725)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for SAR1B Antibody (NBP1-32725) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-32725:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot SAR1B NBP1-32725
Enlarge
5
reviewed by:
Anonymous
WB Mouse 10/23/2019
View

Summary

ApplicationWestern Blot
Sample TestedAdult small intestine
SpeciesMouse
Lot40023

Comments

CommentsMouse small intestine was homogenised and protein content was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody Sar1B diluted 1:1000 in 5% BSA 2, before incubation with anti rabbit secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SAR1B Antibody (NBP1-32725) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional SAR1B Products

Bioinformatics Tool for SAR1B Antibody (NBP1-32725)

Discover related pathways, diseases and genes to SAR1B Antibody (NBP1-32725). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SAR1B Antibody (NBP1-32725)

Discover more about diseases related to SAR1B Antibody (NBP1-32725).
 

Pathways for SAR1B Antibody (NBP1-32725)

View related products by pathway.

PTMs for SAR1B Antibody (NBP1-32725)

Learn more about PTMs related to SAR1B Antibody (NBP1-32725).
 

Research Areas for SAR1B Antibody (NBP1-32725)

Find related products by research area.

Blogs on SAR1B

There are no specific blogs for SAR1B, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
10/23/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SAR1B
Entrez
Uniprot