SAR1B Antibody

Images

 
Western Blot: SAR1B Antibody [NBP1-32725] - A. 50 ug rat brain lysate/extract 12 % SDS-PAGE SAR1B antibody dilution: 1:3000
Immunocytochemistry/ Immunofluorescence: SAR1B Antibody [NBP1-32725] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
Western Blot: SAR1B Antibody [NBP1-32725] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE, antibody diluted at 1:3000.
Western Blot: SAR1B Antibody [NBP1-32725] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE, antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SAR1B Antibody Summary

Immunogen
Full length human SAR1B Recombinant protein.
Localization
Endoplasmic reticulum membrane, Peripheral membrane protein, Golgi apparatus, Golgi stack membrane, Peripheral membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SAR1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Publications
Read Publication using
NBP1-32725 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SAR1B Antibody

  • 2310075M17Rik
  • ANDD
  • CMRD
  • EC 3.6.5
  • GTP-binding protein B
  • GTP-binding protein SAR1b
  • GTP-binding protein Sara
  • SAR1 homolog B (S. cerevisiae)
  • SAR1a gene homolog (S. cerevisiae) 2
  • SAR1a gene homolog 2 (S. cerevisiae)
  • SAR1a gene homolog 2
  • SARA2GTBPB
  • SARB

Background

SAR1B belongs to the small GTPase superfamily, SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus and is activated by the guanine nucleotide exchange factor PREB. SAR1B is involved in the selection of the protein cargo and the assembly of the COPII coat complex. Defects in SAR1B are the cause of chylomicron retention disease (CMRD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The conditions are characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SAR1B Antibody (NBP1-32725)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SAR1B
Entrez
Uniprot