Western Blot: SAR1B Antibody [NBP1-32725] - Adult mouse small intestine homogenate, 20 ug total protein per lane. Antibody at 1:1000. Secondary HRP conjugated antibody at 1:5000. Bands visualized with Immobilon Western ...read more
Immunocytochemistry/ Immunofluorescence: SAR1B Antibody [NBP1-32725] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725] - Mouse ovary. SAR1B antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: SAR1B Antibody [NBP1-32725] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE, antibody diluted at 1:3000.
Western Blot: SAR1B Antibody [NBP1-32725] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE, antibody diluted at 1:1000.
Western Blot: SAR1B Antibody [NBP1-32725] - A. 50 ug rat brain lysate/extract 12 % SDS-PAGE SAR1B antibody dilution: 1:3000
Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
SAR1B antibody validated for WB from a verified customer review.
22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-32725 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Immunogen affinity purified
Alternate Names for SAR1B Antibody
GTP-binding protein B
GTP-binding protein SAR1b
GTP-binding protein Sara
SAR1 homolog B (S. cerevisiae)
SAR1a gene homolog (S. cerevisiae) 2
SAR1a gene homolog 2 (S. cerevisiae)
SAR1a gene homolog 2
SAR1B belongs to the small GTPase superfamily, SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus and is activated by the guanine nucleotide exchange factor PREB. SAR1B is involved in the selection of the protein cargo and the assembly of the COPII coat complex. Defects in SAR1B are the cause of chylomicron retention disease (CMRD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The conditions are characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for SAR1B Antibody (NBP1-32725)(1)
We have publications tested in 1 confirmed species: Human.
Mouse small intestine was homogenised and protein content was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody Sar1B diluted 1:1000 in 5% BSA 2, before incubation with anti rabbit secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.
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