SEC23A Antibody

Western Blot: SEC23A Antibody [NBP2-34842] - Analysis was performed whole cell extracts and tissue extracts of HeLa (Lane 1), PC-12 (Lane 2), NIH/3T3 (Lane 3), Mouse Liver (Lane 4) and Rat Liver (Lane 5).

Immunocytochemistry/Immunofluorescence: SEC23A Antibody [NBP2-34842] - Analysis of COP II (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).

Flow Cytometry: SEC23A Antibody [NBP2-34842] - Analysis of COP II was done on PC-12 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with COP II Rabbit Polyclonal Antibody (PA1-069A, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA.

Immunocytochemistry/Immunofluorescence: SEC23A Antibody [NBP2-34842] - Analysis of COP II (green) showing staining in the cytoplasm of PC12 cells (right) compared to a negative control without primary antibody (left).

Immunocytochemistry/Immunofluorescence: SEC23A Antibody [NBP2-34842] - Analysis of COP II (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).

• Reactivity: Hu, Mu, Rt, Ha
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SEC23A Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEC23A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
  • Flow Cytometry 3 - 5 ug / 10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:200
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: Useful in WB. WB: Detects an approx. 85 kDa protein representing COPII from PC-12 cell extract. May be useful in ICC and IP as reported in scientific literature.

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 6452637).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SEC23A Antibody

• CLSD
• MGC26267
• protein transport protein Sec23A
• Sec23 (S. cerevisiae) homolog A
• Sec23 homolog A (S. cerevisiae)
• SEC23-related protein A

Background

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two Coatomer-Protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEC23A
• Entrez:
  • 26958(Human)
  • 54160(Mouse)
  • 58847(Rat)
• Uniprot:
  • B5DFC3(Rat)