SAFB Antibody (6F7)

Images

 
Western Blot: SAFB Antibody (6F7) [NB200-332] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5% ...read more
Immunocytochemistry/ Immunofluorescence: SAFB Antibody (6F7) [NB200-332] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, antibody at 1:200 dilution.
Western Blot: SAFB Antibody (6F7) [NB200-332] - Detection of human HET SAF-B protein in HeLa whole cell lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
6F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

SAFB Antibody (6F7) Summary

Immunogen
A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human HET protein.
Specificity
This is specific for HET SAF B protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SAFB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation
Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SAFB Antibody (6F7)

  • glutathione S-transferase fusion protein
  • HAP
  • heat-shock protein (HSP27) estrogen response element and TATA box-bindingprotein
  • HETDKFZp779C1727
  • Hsp27 ERE-TATA binding protein
  • HSP27 ERE-TATA-binding protein
  • HSP27 estrogen response element-TATA box-binding protein
  • SAF-B
  • SAF-B1
  • SAFB1SAB-B1
  • scaffold attachment factor B
  • scaffold attachment factor B1

Background

Hsp27 ERE-TATA binding protein (HET)/ Scaffold Attachment Factor-B (SAF-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes. The calculated molecular weight of HET/SAF-B is 102 kDa (Accession No. U72355), but on SDS PAGE gels HET/SAF-B runs as an approximately 130 kDa protein. This aberrant migration is due to a large number of charged amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SAFB Antibody (NB200-332) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-332AF405
Alexa Fluor 488 Labeled NB200-332AF488
Alexa Fluor 647 Labeled NB200-332AF647
Alexa Fluor 700 Labeled NB200-332AF700
DyLight 350 Labeled NB200-332UV
DyLight 405 Labeled NB200-332V
DyLight 488 Labeled NB200-332G
DyLight 680 Labeled NB200-332FR
DyLight 755 Labeled NB200-332IR

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Bioinformatics

Gene Symbol SAFB
Entrez