SAFB Antibody (6F7) - Azide and BSA Free

Western Blot: SAFB Antibody (6F7) [NB200-332] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5% SDS-PAGE SAFB antibody dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: SAFB Antibody (6F7) [NB200-332] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, antibody at 1:200 dilution.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clone: 6F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SAFB Antibody (6F7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SAFB Antibody (6F7) - Azide and BSA Free (NB200-332) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human SAFB protein.
• Specificity: This is specific for HET SAF B protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SAFB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot 1-2 ug/ml
• Application Notes: ICC/IF, IHC, IP- Assay dependent
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for SAFB Antibody (6F7) - Azide and BSA Free

• glutathione S-transferase fusion protein
• HAP
• heat-shock protein (HSP27) estrogen response element and TATA box-bindingprotein
• HETDKFZp779C1727
• Hsp27 ERE-TATA binding protein
• HSP27 ERE-TATA-binding protein
• HSP27 estrogen response element-TATA box-binding protein
• SAF-B
• SAF-B1
• SAFB1SAB-B1
• scaffold attachment factor B
• scaffold attachment factor B1

Background

Hsp27 ERE-TATA binding protein (HET)/ Scaffold Attachment Factor-B (SAF-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes. The calculated molecular weight of HET/SAF-B is 102 kDa (Accession No. U72355), but on SDS PAGE gels HET/SAF-B runs as an approximately 130 kDa protein. This aberrant migration is due to a large number of charged amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAFB
• Entrez:
  • 6294(Human)
  • 224903(Mouse)