SA1 Antibody (8810) - Azide and BSA Free

Western Blot: SA1 Antibody (8810) [NBP2-43750] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [8810].

Immunocytochemistry/Immunofluorescence: SA1 Antibody (8810) [NBP2-43750] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: STAG1 protein stained by STAG1 antibody [8810] diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Western Blot: SA1 Antibody (8810) [NBP2-43750] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with STAG1 antibody diluted by 1:1000.

Immunoprecipitation: SA1 Antibody (8810) [NBP2-43750] - Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 ug of SA1 antibody [8810]. Western blot analysis was performed using SA1 antibody [8810] EasyBlot anti-Mouse IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Xp
• Applications: WB, ICC/IF, IP
• Clone: 8810
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SA1 Antibody (8810) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SA1 Antibody (8810) - Azide and BSA Free (NBP2-43750) is a monoclonal antibody validated for use in WB, ICC/IF and IP. Anti-SA1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SA1. The exact sequence is proprietary.
• Predicted Species: Mouse (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 144 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for SA1 Antibody (8810) - Azide and BSA Free

• cohesin subunit SA-1
• nuclear protein stromal antigen 1
• SA1
• stromal antigen 1

Background

SA1 is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SA1 Antibody (NBP2-43750)(1)

Publication using NBP2-43750

• Gilbertson SR, Zhang N, Lin KF et al. Synthesis and Evaluation of a Class of Compounds Inhibiting the Growth of Stromal Antigen 2 (STAG2)-Mutant Ewing Sarcoma Cells ChemMedChem 2022-01-11 [PMID: 35018729]

Bioinformatics

• Gene Symbol: STAG1