SA1 Antibody (2E9) - Azide and BSA Free

Western Blot: SA1 Antibody (2E9) [H00010274-M01] - STAG1 monoclonal antibody (M01), clone 2E9 Analysis of STAG1 expression in HeLa.

Immunocytochemistry/Immunofluorescence: SA1 Antibody (2E9) [H00010274-M01] - Analysis of monoclonal antibody to STAG1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: SA1 Antibody (2E9) [H00010274-M01] - Analysis of monoclonal antibody to STAG1 on formalin-fixed paraffin-embedded human smooth muscle. Antibody concentration 3 ug/ml.

Sandwich ELISA: SA1 Antibody (2E9) [H00010274-M01] - Detection limit for recombinant GST tagged STAG1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SA1 Antibody (2E9) - Azide and BSA Free Summary

• Immunogen: STAG1 (AAH64699, 1112 a.a. ~ 1221 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDFLHNRGLMEEDAEPIFEDVMMSSRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF
• Specificity: STAG1 - stromal antigen 1
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAG1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SA1 Antibody (2E9) - Azide and BSA Free

• cohesin subunit SA-1
• nuclear protein stromal antigen 1
• SA1
• stromal antigen 1

Background

This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAG1
• Entrez:
  • 10274(Human)
• OMIM:
  • 604358(Human)
• Uniprot:
  • AAH64699(Human)