S100A9 Antibodies: Novus Biologicals

AF2065

S100A9 Antibody [Unconjugated]

(2 Reviews)

100 μg

AF2065

HAF017). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysate of mouse lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of mouse lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counte-rstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

S100A9 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using 10 µg/mL Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counte-rstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

S100A9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

S100A9 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A9 at approximately 20 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

S100A8/A9 proteins augment differentiation and osteoclastic activity derived from iOCPs but not from hOCPs. a Expression of S100A8, S100A9 and RAGE in sorted control and inflamed BM iOCPs and hOCPs. The protein phosphatase 2A catalytic subunit (PP2Ac) is shown as a loading control. b Densitometry measurements of three biological repeats (no statistical analysis presented). c Sorted control and inflamed BM iOCPs and hOCPs (5 × 104) were cultured on the Osteo assay surface with or without anti-RAGE blocking antibodies (bar: 50 µm). d Pit area quantitation. e Sorted iOCPs and hOCPs (5 × 104) from the BM of control mice were cultured on the Osteo assay surface in combination with a recombinant S100A8/A9 heterodimer and anti-RAGE blocking antibodies as indicated (bar: 50 µm). f Pit area quantitation. c–f Depict representative results for two independent experiments, n = 5 for each group. Line: median, box: 25th-75th percentile, whiskers: range. *P < 0.05 (Mann–Whitney test and Holm multiplicity correction) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35396510), licensed under a CC-BY license. Not internally tested by R&D Systems.

iASPP deficient keratinocytes induce pro-inflammatory gene expression in vitro and attract macrophages in vivo(A) qPCR analysis of mRNA expression levels of inflammatory genes (and iASPP gene Ppp1r13l) in iASPP WT and KO primary keratinocytes at 0, 1, and 6 h after treatment with TNF-alpha . Values are mean + SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; n = 3 biological replicates.(B) Left, IHC staining of S100a8 and S100a9 in untreated skin sections from iASPP WT and KO mice (adjacent sections). Scale bar, 50 μm. Right, IB of iASPP and S100a9 expression levels in iASPP WT (−tamoxifen) and KO (+tamoxifen) primary keratinocytes.(C) H&E analysis of acetone- or TPA-treated skin sections from iASPP WT and KO mice. Scale bar, 50 μm. Histograms below show epidermal thickness in the same samples. Values are mean ± SD. n = 3 (WT) and n = 4 (KO) mice in acetone cohort; n = 4 (WT), n = 3 (KO) mice in TPA cohort.(D) IHC staining of F4/80-positive macrophages in acetone- or TPA-treated skin sections from iASPP WT and KO mice. Scale bar, 50 μm. Histograms below show quantification of the same samples. Values are mean + SD. Same cohort as in (C).See STAR Methods for p calculations for (A), (C), and (D). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36261000), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2065U-100UG
Alexa Fluor 405	AF2065V-100UG
Alexa Fluor 488	AF2065G-100UG
Alexa Fluor 532	AF2065X-100UG
Alexa Fluor 594	AF2065T-100UG
Alexa Fluor 647	AF2065R-100UG
Alexa Fluor 700	AF2065N-100UG
Alexa Fluor 750	AF2065S-100UG
Alexa Fluor Plus 405	AF2065AFP405-100UG
Alexa Fluor Plus 488	AF2065AFP488-100UG
Alexa Fluor Plus 555	AF2065AFP555-100UG
Alexa Fluor Plus 594	AF2065AFP594-100UG
Alexa Fluor Plus 647	AF2065AFP647-100UG
Alexa Fluor Plus 680	AF2065AFP680-100UG
Allophycocyanin	AF2065A
Biotin	AF2065B
CoraFluor 1	AF2065CL1
DyLight 350	AF2065D
DyLight 405	AF2065E
DyLight 488	AF2065K
DyLight 550	AF2065L
DyLight 594	AF2065M
DyLight 650	AF2065W
DyLight 680	AF2065Y
DyLight 755	AF2065Z
FITC	AF2065F
HRP	AF2065H
Janelia Fluor 525	AF2065JF525
Janelia Fluor 549	AF2065I
Janelia Fluor 646	AF2065J
Janelia Fluor 669	AF2065JF669
PE	AF2065P
PerCP	AF2065C
mFluor Violet 450 SE	AF2065MFV450
mFluor Violet 500 SE	AF2065MFV500
mFluor Violet 610 SE	AF2065MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2065-SP

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NB110-89726

S100A9 Antibody - BSA Free

(27 Publications)

0.1 ml

NB110-89726

<b>Biological Strategies Validation. </b>S100A9-Antibody-Immunohistochemistry-Paraffin-NB110-89726-img0010.jpg

<b>Biological Strategies Validation. </b>Western Blot: S100A9 Antibody [NB110-89726] - Analysis of S100A9 Antibody in DMSO treated HL60 whole cell lysates.

Simple Western: S100A9 Antibody [NB110-89726] - Simple Western lane view shows a specific band for S100A9 in 0.5 mg/ml of Human PBMC's lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, & (F) Cxcl5 in normal control, IgG Ab, & anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: S100A9 Antibody [NB110-89726] - Effects of anti-S100a9 Ab administration on the azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colitis-associated cancer development. (A) Experimental procedure of the control group & the AOM/DSS group treated with IgG Ab or anti-S100a9 Ab. (B) DAI of the IgG Ab or anti-S100a9 Ab-treated AOM/DSS mice & normal controls. (C) General observation of the colorectums in mice at the end of the 13th & 18th week. (D) Colon length, tumor rate, & number of macroscopic neoplasms were statisticed at 13 & 18 weeks, individually. n = 5–9 per group. Results were representative of the three experiments performed. (E) Histopathological examination of colon sections was shown under the Pannoramic Viewer (H&E staining, upper panels: original magnification 10×, scale bar: 2000 µm; lower panels: original magnification 200×, scale bar: 100 µm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: S100A9 Antibody [NB110-89726] - Effects of anti-S100a9 Ab on the frequency of neutrophils, macrophages, & dendritic cells (DCs) in the colon of the dextran sulfate sodium (DSS) mouse model. (A) Colon lamina propria cells were isolated from normal control & IgG Ab or anti-S100a9 Ab-treated DSS mice at day 6 post-DSS colitis induction. Frequencies of neutrophils, macrophages, & DCs in the colon were determined by flow cytometry. Cells were gated on CD45+CD3−CD4−CD11b+Ly6G+, CD45+CD3−CD4−CD11b+F4/80+, & CD45+CD3−CD4−CD11b+CD11c+ populations respectively. Representative flow cytometric figures were shown. The percentage of cells was presented as the mean ± SEM of four to six individual mice per group. *p < 0.05 in a one-way analysis of variance followed by Bonferroni correction. Data were representative of three independent experiments. (B) Immunohistochemical staining of myeloperoxidase (MPO), CD68, & CD11c proteins in the normal control & IgG Ab or anti-S100a9 Ab-treated colitis mice at day 6 (left panels: original magnification 40×, scale bar: 200 µm; right panels: original magnification 200×, scale bar: 50 µm). Staining scores were counted. One-way analysis of variance followed by Bonferroni correction. Results were representative of the three experiments performed. Error bars represent SD. (C) Expression of S100a9, Tnf alpha , Il1 beta , Il6, Il17a, Ifn gamma , Il12a, Il23a, Il4, & Il10 mRNA, as assessed by quantitative real-time PCR in normal control & IgG Ab, or anti-S100a9 Ab-treated colitis tissues. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: S100A9 Antibody [NB110-89726] - Anti-S100a9 Ab ameliorates inflammatory response of dextran sulfate sodium (DSS)-induced colitis in mice. (A) 6 days after DSS treatment, representative H&E-stained colon sections were shown (upper panels: original magnification 40×, scale bar: 200 µm; lower panels: original magnification 100×, scale bar: 100 µm). (B) Colon inflammation, ulceration, & crypt damage were scored individually, & composite total score was scored. n = 5 per group. (C) Isolated lymphoid follicles (ILFs) area was measured at day 6. Representative TUNEL staining (D) & ethynyl-2′-deoxyuridine (EdU) staining (E) of normal mice & DSS-induced mice, which were treated with IgG Ab or anti-S100a9 Ab on day 6. The percent of positive cells was measured. At least six fields were counted per mouse. Scale bar, 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: S100A9 Antibody [NB110-89726] - Host CP (S100A8/A9) is elevated in H. pylori infected stomach tissue.A) s100a8/s100a9 transcript abundance in RNA extracted from C57BL/6 mice infected with H. pylori PMSS1 or SS1 for 1, 2, or 3 months relative to uninfected animals as determined by real-time RT-PCR analysis. Points indicate mean relative units of transcript abundance +/− SEM (levels of s100a8 in PMSS1-infected mice compared to uninfected mice; 1 mo p = 0.0511; 2 mo p = 0.0432; 3 mo p = 0.0127; levels of s100a8 in SS1-infected mice compared to uninfected mice at 2 mo p = 0.0623 Student's t test). (B) Inflammation scores of H. pylori infected mice at 1, 2, & 3 months post infection. (C) s100a8/s100a9 transcript abundance in RNA extracted from gastric biopsies derived from human patients, which were either H. pylori-positive or H. pylori-negative (s100a8 p = 0.15; s100a9 *p = 0.05). Bars indicate mean relative units of transcript abundance +/− SEM. Each experimental group represents≥5 individuals (mice or human samples). D) Gastric samples derived from H. pylori PMSS1-infected WT mice or SS1-infected WT mice at 2 months post-infection were analyzed via immunohistochemistry using a polyclonal antibody to S100A9 (scale bars are 50 microns). E) Real-time RT-PCR was performed on gastric tissue to quantify s100a8 & s100a9 transcript abundance from WT (C57BL/6 mice) & IL-17RA-/- mice infected with PMSS1. Data represent relative units of transcript abundance +/− SEM in WT mice & IL-17RA-/- mice, *p = 0.0169 & p = 0.0143, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25330071), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-89726SS

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MAB2065

S100A9 Antibody (372510) [Unconjugated]

(11 Publications)

100 μg

MAB2065

HAF005). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse leukocytes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (<a class=

HAF005). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse leukocytes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A9 at approximately 19 kDa (as indicated) using 10 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2065U-100UG
Alexa Fluor 405	FAB2065V-100UG
Alexa Fluor 488	FAB2065G-100UG
Alexa Fluor 532	FAB2065X-100UG
Alexa Fluor 594	FAB2065T-100UG
Alexa Fluor 647	FAB2065R-100UG
Alexa Fluor 700	FAB2065N-100UG
Alexa Fluor 750	FAB2065S-100UG
Alexa Fluor Plus 405	FAB2065AFP405-100UG
Alexa Fluor Plus 488	FAB2065AFP488-100UG
Alexa Fluor Plus 555	FAB2065AFP555-100UG
Alexa Fluor Plus 594	FAB2065AFP594-100UG
Alexa Fluor Plus 647	FAB2065AFP647-100UG
Alexa Fluor Plus 680	FAB2065AFP680-100UG
Allophycocyanin	FAB2065A
Biotin	FAB2065B
CoraFluor 1	FAB2065CL1
DyLight 350	FAB2065D
DyLight 405	FAB2065E
DyLight 488	FAB2065K
DyLight 550	FAB2065L
DyLight 594	FAB2065M
DyLight 650	FAB2065W
DyLight 680	FAB2065Y
DyLight 755	FAB2065Z
FITC	FAB2065F
HRP	FAB2065H
Janelia Fluor 525	FAB2065JF525
Janelia Fluor 549	FAB2065I
Janelia Fluor 646	FAB2065J
Janelia Fluor 669	FAB2065JF669
PE	FAB2065P
PerCP	FAB2065C
mFluor Violet 450 SE	FAB2065MFV450
mFluor Violet 500 SE	FAB2065MFV500
mFluor Violet 610 SE	FAB2065MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2065-SP

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NBP2-45295

S100A9 Antibody (MAC387)

(3 Publications)

0.1 mg

NBP2-45295

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Gp, Gt, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Gp, Gt, Pm, Rb

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-47667AF350
Alexa Fluor 405	NBP2-47667AF405
Alexa Fluor 488	NBP2-47667AF488
Alexa Fluor 532	NBP2-47667AF532
Alexa Fluor 594	NBP2-47667AF594
Alexa Fluor 647	NBP2-47667AF647
Alexa Fluor 700	NBP2-47667AF700
Alexa Fluor 750	NBP2-47667AF750
Alexa Fluor Plus 405	NBP2-47667AFP405
Alexa Fluor Plus 488	NBP2-47667AFP488
Alexa Fluor Plus 555	NBP2-47667AFP555
Alexa Fluor Plus 594	NBP2-47667AFP594
Alexa Fluor Plus 647	NBP2-47667AFP647
Alexa Fluor Plus 680	NBP2-47667AFP680
Allophycocyanin	NBP2-47667APC
Biotin	NBP2-47667B
CoraFluor 1	NBP2-47667CL1
DyLight 350	NBP2-47667UV
DyLight 405	NBP2-47667V
DyLight 488	NBP2-47667G
DyLight 550	NBP2-47667R
DyLight 594	NBP2-47667DL594
DyLight 650	NBP2-47667C
DyLight 680	NBP2-47667FR
DyLight 755	NBP2-47667IR
FITC	NBP2-47667F
HRP	NBP2-47667H
Janelia Fluor 525	NBP2-47667JF525
Janelia Fluor 549	NBP2-47667JF549
Janelia Fluor 646	NBP2-47667JF646
Janelia Fluor 669	NBP2-47667JF669
PE	NBP2-47667PE
PerCP	NBP2-47667PCP
mFluor Violet 450 SE	NBP2-47667MFV450
mFluor Violet 500 SE	NBP2-47667MFV500
mFluor Violet 610 SE	NBP2-47667MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-47667-0.1mg

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AF5578

S100A9 Antibody [Unconjugated]

100 μg

AF5578

HAF016). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC), human spleen tissue, human tonsil tissue, and human cartilage tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5578) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human peripheral blood mononuclear cells (PBMC), human spleen tissue, human tonsil tissue, and human cartilage tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5578) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

S100A9 was detected in immersion fixed MDA-MB-468 human breast cancer cell line using Sheep Anti-Human S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5578) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of chondrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

S100A9 was detected in immersion fixed paraffin-embedded sections of human cartilage using Sheep Anti-Human S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5578) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of chondrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF016). A specific band was detected for S100A9 at approximately 14 kDa (as indicated) in the parental MDA-MB-468 cell line, but is not detectable in knockout MDA-MB-468 cell line. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of MDA-MB-468 human breast cancer parental cell line and S100A9 knock out MDA-MB-468 cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5578) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for S100A9 at approximately 14 kDa (as indicated) in the parental MDA-MB-468 cell line, but is not detectable in knockout MDA-MB-468 cell line. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

FAB3832P) and either (A) Sheep Anti-Human S100A9 Polyclonal Antibody (Catalog # AF5578) or (B) Sheep IgG Isotype Control (Catalog # 5-001-A) followed by anti-Sheep IgG APC-conjugated Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer (Catalog # FC005). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

Human peripheral blood monocytes (PBMC) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # <a class=

FAB3832P) and either (A) Sheep Anti-Human S100A9 Polyclonal Antibody (Catalog # AF5578) or (B) Sheep IgG Isotype Control (Catalog # 5-001-A) followed by anti-Sheep IgG APC-conjugated Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer (Catalog # FC005). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Genetic Strategies Validation. </b>S100A9 was detected in immersion fixed MDA-MB-468 human breast cancer cell line but is not detected in S100A9 knockout (KO) MDA-MB-468 cell line using Sheep Anti-Human S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5578) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF5578U-100UG
Alexa Fluor 405	AF5578V-100UG
Alexa Fluor 488	AF5578G-100UG
Alexa Fluor 532	AF5578X-100UG
Alexa Fluor 594	AF5578T-100UG
Alexa Fluor 647	AF5578R-100UG
Alexa Fluor 700	AF5578N-100UG
Alexa Fluor 750	AF5578S-100UG
Alexa Fluor Plus 405	AF5578AFP405-100UG
Alexa Fluor Plus 488	AF5578AFP488-100UG
Alexa Fluor Plus 555	AF5578AFP555-100UG
Alexa Fluor Plus 594	AF5578AFP594-100UG
Alexa Fluor Plus 647	AF5578AFP647-100UG
Alexa Fluor Plus 680	AF5578AFP680-100UG
Allophycocyanin	AF5578A
Biotin	AF5578B
CoraFluor 1	AF5578CL1
DyLight 350	AF5578D
DyLight 405	AF5578E
DyLight 488	AF5578K
DyLight 550	AF5578L
DyLight 594	AF5578M
DyLight 650	AF5578W
DyLight 680	AF5578Y
DyLight 755	AF5578Z
FITC	AF5578F
HRP	AF5578H
Janelia Fluor 525	AF5578JF525
Janelia Fluor 549	AF5578I
Janelia Fluor 646	AF5578J
Janelia Fluor 669	AF5578JF669
PE	AF5578P
PerCP	AF5578C
mFluor Violet 450 SE	AF5578MFV450
mFluor Violet 500 SE	AF5578MFV500
mFluor Violet 610 SE	AF5578MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5578-SP

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NBP1-89360

S100A9 Antibody - BSA Free

0.1 ml

NBP1-89360

<b>Orthogonal Strategies Validation. </b>Staining of human esophagus shows moderate to strong cytoplasmic and nuclear positivity in squamous epithelial cells.

Staining of human stomach shows no positivity in glandular cells as expected.

Staining of human skin shows moderate to strong cytoplasmic and nuclear positivity in squamous epithelial cells.

Staining of human bone marrow shows strong cytoplasmic positivity in a subset of hematopoietic cells.

Simple Western: S100A9 Antibody [NBP1-89360] - Electropherogram image(s) of corresponding Simple Western lane view. S100A9 antibody was used at 1:20 dilution on h. Tonsil lysate(s).

Simple Western: S100A9 Antibody [NBP1-89360] - Simple Western lane view shows a specific band for S100A9 in 0.2 mg/ml of h. Tonsil lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89360-25ul

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MAB5578

S100A9 Antibody (474315) [Unconjugated]

(2 Publications)

100 μg

MAB5578

HAF018). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF018). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human peripheral blood mononuclear cells (PBMC), human spleen tissue, human tonsil tissue, and human cartilage tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human S100A9 Monoclonal Antibody (Catalog # MAB5578) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

S100A9 was detected in immersion fixed Capan-1 human pancreatic adenocarcinoma cell line using 10 µg/mL Mouse Anti-Human S100A9 Monoclonal Antibody (Catalog # MAB5578) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB5578U-100UG
Alexa Fluor 405	FAB5578V-100UG
Alexa Fluor 488	FAB5578G-100UG
Alexa Fluor 532	FAB5578X-100UG
Alexa Fluor 594	FAB5578T-100UG
Alexa Fluor 647	FAB5578R-100UG
Alexa Fluor 700	FAB5578N-100UG
Alexa Fluor 750	FAB5578S-100UG
Alexa Fluor Plus 405	FAB5578AFP405-100UG
Alexa Fluor Plus 488	FAB5578AFP488-100UG
Alexa Fluor Plus 555	FAB5578AFP555-100UG
Alexa Fluor Plus 594	FAB5578AFP594-100UG
Alexa Fluor Plus 647	FAB5578AFP647-100UG
Alexa Fluor Plus 680	FAB5578AFP680-100UG
Allophycocyanin	FAB5578A
Biotin	FAB5578B
CoraFluor 1	FAB5578CL1
DyLight 350	FAB5578D
DyLight 405	FAB5578E
DyLight 488	FAB5578K
DyLight 550	FAB5578L
DyLight 594	FAB5578M
DyLight 650	FAB5578W
DyLight 680	FAB5578Y
DyLight 755	FAB5578Z
FITC	FAB5578F
HRP	FAB5578H
Janelia Fluor 525	FAB5578JF525
Janelia Fluor 549	FAB5578I
Janelia Fluor 646	FAB5578J
Janelia Fluor 669	FAB5578JF669
PE	FAB5578P
PerCP	FAB5578C
mFluor Violet 450 SE	FAB5578MFV450
mFluor Violet 500 SE	FAB5578MFV500
mFluor Violet 610 SE	FAB5578MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB5578-SP

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NBP2-45662

S100A9 Antibody (OTI10D5)

0.1 ml

NBP2-45662

Immunohistochemistry: S100A9 Antibody (10D5) [NBP2-45662] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: S100A9 Antibody (10D5) [NBP2-45662] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: S100A9 Antibody (10D5) [NBP2-45662] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: S100A9 Antibody (10D5) [NBP2-45662] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: S100A9 Antibody (10D5) [NBP2-45662] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73970AF350
Alexa Fluor 405	NBP2-73970AF405
Alexa Fluor 488	NBP2-73970AF488
Alexa Fluor 532	NBP2-73970AF532
Alexa Fluor 594	NBP2-73970AF594
Alexa Fluor 647	NBP2-73970AF647
Alexa Fluor 700	NBP2-73970AF700
Alexa Fluor 750	NBP2-73970AF750
Alexa Fluor Plus 405	NBP2-73970AFP405
Alexa Fluor Plus 488	NBP2-73970AFP488
Alexa Fluor Plus 555	NBP2-73970AFP555
Alexa Fluor Plus 594	NBP2-73970AFP594
Alexa Fluor Plus 647	NBP2-73970AFP647
Alexa Fluor Plus 680	NBP2-73970AFP680
Allophycocyanin	NBP2-73970APC
Biotin	NBP2-73970B
CoraFluor 1	NBP2-73970CL1
DyLight 350	NBP2-73970UV
DyLight 405	NBP2-73970V
DyLight 488	NBP2-73970G
DyLight 550	NBP2-73970R
DyLight 594	NBP2-73970DL594
DyLight 650	NBP2-73970C
DyLight 680	NBP2-73970FR
DyLight 755	NBP2-73970IR
FITC	NBP2-73970F
HRP	NBP2-73970H
Janelia Fluor 525	NBP2-73970JF525
Janelia Fluor 549	NBP2-73970JF549
Janelia Fluor 646	NBP2-73970JF646
Janelia Fluor 669	NBP2-73970JF669
PE	NBP2-73970PE
PerCP	NBP2-73970PCP
mFluor Violet 450 SE	NBP2-73970MFV450
mFluor Violet 500 SE	NBP2-73970MFV500
mFluor Violet 610 SE	NBP2-73970MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73970

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NBP3-07983

S100A9 Antibody (CL11191) - BSA Free

(1 Publication)

100 ul

NBP3-07983

Immunohistochemistry-Paraffin: S100A9 Antibody (CL11191) [NBP3-07983] - Staining of human bone marrow shows moderate to strong cytoplasmic positivity in hematopoietic cells.

Immunohistochemistry-Paraffin: S100A9 Antibody (CL11191) [NBP3-07983] - Staining of human kidney shows no positivity in cells in tubules as expected.

Immunohistochemistry-Paraffin: S100A9 Antibody (CL11191) [NBP3-07983] - Staining of human tonsil shows strong cytoplasmic positivity in squamous epithelial cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43896AF350
Alexa Fluor 405	NBP3-43896AF405
Alexa Fluor 488	NBP3-43896AF488
Alexa Fluor 532	NBP3-43896AF532
Alexa Fluor 594	NBP3-43896AF594
Alexa Fluor 647	NBP3-43896AF647
Alexa Fluor 700	NBP3-43896AF700
Alexa Fluor 750	NBP3-43896AF750
Alexa Fluor Plus 405	NBP3-43896AFP405
Alexa Fluor Plus 488	NBP3-43896AFP488
Alexa Fluor Plus 555	NBP3-43896AFP555
Alexa Fluor Plus 594	NBP3-43896AFP594
Alexa Fluor Plus 647	NBP3-43896AFP647
Alexa Fluor Plus 680	NBP3-43896AFP680
Allophycocyanin	NBP3-43896APC
Biotin	NBP3-43896B
CoraFluor 1	NBP3-43896CL1
DyLight 350	NBP3-43896UV
DyLight 405	NBP3-43896V
DyLight 488	NBP3-43896G
DyLight 550	NBP3-43896R
DyLight 594	NBP3-43896DL594
DyLight 650	NBP3-43896C
DyLight 680	NBP3-43896FR
DyLight 755	NBP3-43896IR
FITC	NBP3-43896F
HRP	NBP3-43896H
Janelia Fluor 525	NBP3-43896JF525
Janelia Fluor 549	NBP3-43896JF549
Janelia Fluor 646	NBP3-43896JF646
Janelia Fluor 669	NBP3-43896JF669
PE	NBP3-43896PE
PerCP	NBP3-43896PCP
mFluor Violet 450 SE	NBP3-43896MFV450
mFluor Violet 500 SE	NBP3-43896MFV500
mFluor Violet 610 SE	NBP3-43896MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43896
BSA Free	NBP3-07983-25ul

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NBP2-44913

S100A9 Antibody (S100A9/1075) - BSA Free

0.1 mg

NBP2-44913

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-11559AF350
Alexa Fluor 405	NBP3-11559AF405
Alexa Fluor 488	NBP3-11559AF488
Alexa Fluor 532	NBP3-11559AF532
Alexa Fluor 594	NBP3-11559AF594
Alexa Fluor 647	NBP3-11559AF647
Alexa Fluor 700	NBP3-11559AF700
Alexa Fluor 750	NBP3-11559AF750
Alexa Fluor Plus 405	NBP3-11559AFP405
Alexa Fluor Plus 555	NBP3-11559AFP555
Alexa Fluor Plus 594	NBP3-11559AFP594
Alexa Fluor Plus 647	NBP3-11559AFP647
Alexa Fluor Plus 680	NBP3-11559AFP680
Allophycocyanin	NBP3-11559APC
Biotin	NBP3-11559B
CoraFluor 1	NBP3-11559CL1
DyLight 350	NBP3-11559UV
DyLight 405	NBP3-11559V
DyLight 488	NBP3-11559G
DyLight 550	NBP3-11559R
DyLight 594	NBP3-11559DL594
DyLight 650	NBP3-11559C
DyLight 680	NBP3-11559FR
DyLight 755	NBP3-11559IR
FITC	NBP3-11559F
HRP	NBP3-11559H
Janelia Fluor 525	NBP3-11559JF525
Janelia Fluor 549	NBP3-11559JF549
Janelia Fluor 646	NBP3-11559JF646
Janelia Fluor 669	NBP3-11559JF669
PE	NBP3-11559PE
PerCP	NBP3-11559PCP
mFluor Violet 450 SE	NBP3-11559MFV450
mFluor Violet 500 SE	NBP3-11559MFV500
mFluor Violet 610 SE	NBP3-11559MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-44913-0.02mg
Azide and BSA Free	NBP3-11559

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NBP3-48972

S100A9 Antibody (rMAC3781)

100 μg

NBP3-48972

Formalin-fixed, paraffin-embedded human spleen stained with S100A9 Antibody (rMAC3781).

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Gp, Gt, Pm, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Gp, Gt, Pm, Pm, Rb

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-49506

S100A9 Antibody (rMAC3781) - Azide and BSA Free

100 μg

NBP3-49506

Formalin-fixed, paraffin-embedded human spleen stained with S100A9 Antibody (rMAC3781) - Azide and BSA Free.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Gp, Gt, Pm, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Gp, Gt, Pm, Pm, Rb

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

S100A9 Antibodies

S100A9 Antibody [Unconjugated]

S100A9 Antibody - BSA Free

S100A9 Antibody (372510) [Unconjugated]

S100A9 Antibody (MAC387)

S100A9 Antibody [Unconjugated]

S100A9 Antibody - BSA Free

S100A9 Antibody (474315) [Unconjugated]

S100A9 Antibody (OTI10D5)

S100A9 Antibody (CL11191) - BSA Free

S100A9 Antibody (S100A9/1075) - BSA Free

S100A9 Antibody (rMAC3781)

S100A9 Antibody (rMAC3781) - Azide and BSA Free