50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Please see the vial label for concentration. If unlisted please contact technical services.
In vitro assay
Useful in Western Blot and ELISA. In vitro assay was reported in scientific literature. This protein has not been tested for any functionality. This product may contain endotoxins and is not suitable for use with live cells.
FAQs for S100A8 Recombinant Protein (H00006279-P01). (Showing 1 - 2 of 2 FAQs).
Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?
Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.
We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.