S100A6 Antibody

Images

 
Western Blot: S100A6 Antibody [NB110-93274] - Detection of S100A6 antibody in Hela whole cell extracts using NB110-93274.
Immunocytochemistry/ Immunofluorescence: S100A6 Antibody [NB110-93274] - S100AG antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

S100A6 Antibody Summary

Immunogen
Full length human S100A6 protein [Swiss-Prot# P06703] expressed in E. coli.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
S100A6
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This S100A6 antibody is useful for Western blot, where a band is seen at ~9 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
S100A6 Lysate (NBL1-15658)
HeLa Lysate (NB820-59462)

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for S100A6 Antibody

  • 2A9
  • CABP
  • CACY
  • CACY5B10
  • calcyclin
  • Growth factor-inducible protein 2A9
  • MLN 4
  • PRA
  • PRAS100 calcium binding protein A6 (calcyclin)
  • Prolactin receptor-associated protein
  • protein S100-A6
  • S100 calcium binding protein A6
  • S100 calcium-binding protein A6 (calcyclin)
  • S100 calcium-binding protein A6
  • S100A6

Background

S100A6 (S100 calcium-binding protein A6) belongs to S-100 family, a family of calcium-binding proteins responsible for regulation of cytoskeletal dynamics, cell proliferation and differentiation. S100A6 protein is a 10-kD polypeptide that localize to nuclear envelope, cytoplasm and cell membranes, and exists as homodimer which interacts with CACYBP, ANXA2, ANXA11, SUGT1, TP53, tropomyosin, FKBP4, CACYBP etc. S100A6 has been suggested to function as calcium sensor and is essential to cellular calcium signaling. S100A6 can also function by interacting with other proteins and indirectly play a role in the reorganization of actin cytoskeleton as well as in cell motility. S100A6 is implicated in regulation of different cellular phenomena such as proliferation, differentiation and apoptosis, and has been associated with tumorigenesis, invasion and/or metastasis. Originally identified in murine Ehrlich ascites tumour cells, S100A6 now known to express at elevated levels in different tumours such as oral squamous cell carcinoma, colorectal adenocarcinoma, cholangiocarcinoma, neuroblastoma, as well as lung, gastric, pancreatic, breast and thyroid cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for S100A6 Antibody (NB110-93274): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S100A6 Antibody (NB110-93274). (Showing 1 - 1 of 1 FAQ).

  1. Our customer needs to biotinylate catalog # NB110-93274, lot # A191664, and is asking for the buffer and the concentration. Can you please provide?
    • NB110-93274 is supplied as whole antiserum with 0.1% sodium azide. As it is purified, the exact concentration of antibody is not quantifiable.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for S100A6 Antibody (NB110-93274)

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Blogs on S100A6.

S100A6: Playing Roles in Cancer, Apoptosis & Transcription Regulation
S100A6 antibodies detect a small calcium binding protein with 2 EF-hand structures and belongs to the S100 family. Calcium binding induces a conformational change of the protein which in turn permits its interaction with several target proteins. It is...  Read full blog post.

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Bioinformatics

Gene Symbol S100A6
Entrez
Uniprot