S100A6 Antibody - BSA Free

Western Blot: S100A6 Antibody [NB110-93274] - Detection of S100A6 antibody in Hela whole cell extracts using NB110-93274.

Immunocytochemistry/Immunofluorescence: S100A6 Antibody [NB110-93274] - S100AG antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

S100A6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit S100A6 Antibody - BSA Free (NB110-93274) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human S100A6 protein [Swiss-Prot# P06703] expressed in E. coli.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: S100A6
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Western Blot 1:1000
• Application Notes: In Western blot, where a band is seen at ~9 kDa.
• Theoretical MW: 9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  S100A6 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for S100A6 Antibody - BSA Free

• 2A9
• CABP
• CACY
• CACY5B10
• calcyclin
• Growth factor-inducible protein 2A9
• MLN 4
• PRA
• PRAS100 calcium binding protein A6 (calcyclin)
• Prolactin receptor-associated protein
• protein S100-A6
• S100 calcium binding protein A6
• S100 calcium-binding protein A6 (calcyclin)
• S100 calcium-binding protein A6
• S100A6

Background

S100A6 (S100 calcium-binding protein A6) belongs to S-100 family, a family of calcium-binding proteins responsible for regulation of cytoskeletal dynamics, cell proliferation and differentiation. S100A6 protein is a 10-kD polypeptide that localize to nuclear envelope, cytoplasm and cell membranes, and exists as homodimer which interacts with CACYBP, ANXA2, ANXA11, SUGT1, TP53, tropomyosin, FKBP4, CACYBP etc. S100A6 has been suggested to function as calcium sensor and is essential to cellular calcium signaling. S100A6 can also function by interacting with other proteins and indirectly play a role in the reorganization of actin cytoskeleton as well as in cell motility. S100A6 is implicated in regulation of different cellular phenomena such as proliferation, differentiation and apoptosis, and has been associated with tumorigenesis, invasion and/or metastasis. Originally identified in murine Ehrlich ascites tumour cells, S100A6 now known to express at elevated levels in different tumours such as oral squamous cell carcinoma, colorectal adenocarcinoma, cholangiocarcinoma , neuroblastoma , as well as lung, gastric, pancreatic, breast and thyroid cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for S100A6 Antibody (NB110-93274). (Showing 1 - 1 of 1 FAQ).

1. Our customer needs to biotinylate catalog # NB110-93274, lot # A191664, and is asking for the buffer and the concentration. Can you please provide?
   • NB110-93274 is supplied as whole antiserum with 0.1% sodium azide. As it is purified, the exact concentration of antibody is not quantifiable.

Bioinformatics

• Gene Symbol: S100A6
• Entrez:
  • 6277(Human)
  • 6277(Human)
  • 85247(Rat)
• Uniprot:
  • P06703()