S100A13 Antibody

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Western blot shows lysates of MCF‑7 human breast cancer cell line. Gels were loaded with 25 µg of cytoplasmic (Cyto) extracts. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human S100A13 Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

S100A13 Antibody Summary

Immunogen
E. coli-derived recombinant human S100A13
Ala2-Lys98
Accession # Q99584
Specificity
Detects human S100A13 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse S100A13 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
S100A13
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Control
S100A13 Knockout 293T Cell Lysate

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A13 Antibody

  • S100 calcium binding protein A13
  • S100 calcium-binding protein A13protein S100-A13
  • S100A13

Background

S100A13 is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of vertebrate EF-hand Ca++-binding proteins (1‑3). It is widely expressed as a homodimer with two 98 amino acid (aa) long subunits (2, 3). Human S100A13 shares 83%, 90%, 91%, 87%, 78% and 47% aa identity with mouse, rat, cow, dog, opossum and chicken S100A13, respectively. Like other S100 proteins, S100A13 is small and generally acidic, but contains a basic residue-rich sequence at the C terminus, and two EF hand motifs that bind with Ca++ differing affinities (2‑4). Some S100 proteins, including S100A13, are able to bind the cell surface receptor for advanced glycation end-products (RAGE) (5). Despite lacking a signal sequence, S100A13 plays an important role in Cu++-dependent export of FGF-1 (FGF acidic) and IL-1 alpha from the cell in response to stresses such as heat shock, anoxia and starvation (6‑8). Binding of copper is necessary for formation of a multi-protein complex between S100A13, FGF-1 and p40 synaptotagmin-1 (syt-1) (9, 10). Cu++ ions supplied by S100A13 are thought to oxidize and downregulate the activity of FGF-1 prior to export (10). Calcium influx may also play a similar role in FGF-1 release from neuronal cells (11). S100A13 is composed of four amphiphilic helices that may interact with acidic phospholipid headgroups. With FGF-1 and syt-1, S100A13 likely perturbs the membrane, which allows the S100A13 protein complex to exit the cell (4, 12). S100A13 has been proposed as a marker for angiogenesis in tumors and endometrium, due to its role in stress-induced export of FGF-1 (13, 14). Based on in house studies, S100A13 has also been found to promote neurite outgrowth from rat cortical embryonic neurons (15).

  1. Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.  
  2. Wicki, R. et al. (1996) Biochem. Biophys. Res. Commun. 227:594. 
  3. Ridinger, K. et al. (2000) J. Biol. Chem. 275:8686. 
  4. Li, M. et al. (2007) Biochem. Biophys. Res. Commun. 356:616. 
  5. Hsieh, H.-L. et al. (2004) Biochem. Biophys. Res. Commun. 316:949. 
  6. Landriscina, M. et al. (2001) J. Biol. Chem. 276:22544. 
  7. Sivaraja, V. et al. (2006) Biophys. J. 91:1832.
  8. Mandinova, A. et al. (2003) J. Cell Sci. 116:2687.
  9. Prudovsky, I. et al. (2002) J. Cell Biol. 158:201.
  10. Landriscina, M. et al. (2001) J. Biol. Chem. 276:25549.
  11. Matsunaga, H. and H. Ueda (2006) Cell. Mol. Neurobiol. 26:237.
  12. Graziani, I. et al. (2006) Biochem. Biophys. Res. Commun. 349:192.
  13. Landriscina, M. et al. (2006) J. Neurooncol. 80:251.
  14. Hayrabedyan, S. et al. (2005) Reprod. Biol. 5:51.
  15. R&D Sytems (2007) In-house data.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol S100A13
Uniprot
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