S-arrestin Antibody (PDS-1)

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Western Blot: S-arrestin Antibody (PDS-1) [NB100-2385] - Western blot analysis of Visual Arrestin expression in human retina tissue extract.

Product Details

Summary
Reactivity Hu, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clone
PDS-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.77 mg/ml

S-arrestin Antibody (PDS-1) Summary

Immunogen
Porcine retinal S-arrestin [Swiss-Prot# P79260]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SAG
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Frozen 1:100-1:200
Application Notes
This S-arrestin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot and the immunopurification of arrestin (PMID 3549614).
Publications
Read Publication using NB100-2385.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
0.77 mg/ml
Purity
Protein G purified

Alternate Names for S-arrestin Antibody (PDS-1)

  • arrestin 1
  • RP47,48 kDa protein
  • S-antigen; retina and pineal gland (arrestin)
  • S-arrestin
  • visual arrestin

Background

Arrestins family contains four types of soluble cytoplasmic proteins that act as negative regulators of GPCR signaling, wherein, cognate ligand binding stimulates GPCR phosphorylation, followed by arrestin binding to phospho-GPCR leading to receptor internalization and ultimately, the desensitization of GPCR signaling. Among four different types of mammalian arrestins, the visual proteins namely arrestin 1 (S-arrestin, S-antigen or SAG) and arrestin 4 (X-arrestin) are localized to retinal rods and cones, respectively, whereas the non-visual proteins arrestin 2 (beta-arrestin 1) and arrestin 3 (beta-arrestin 2) are ubiquitously expressed. S-arrestin is rod specific and constitutes major proteins of retinal rod outer segments and associate with photoactivated/phosphorylated rhodopsin, thereby preventing transducin-mediated phosphodiesterase activation. Besides retinal rod photoreceptor cell outer segment, S-arrestin is also expressed in the pineal gland where its function is largely unknown. Arrestin knockout mice develop photoreceptor cell degeneration because of continuous phototransduction upregulation, as a consequence of defective rhodopsin/opsin shut-off mechanism, and in human subjects, S-arrestin mutations have been linked to Oguchi disease and retinitis pigmentosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S-arrestin Antibody (NB100-2385)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S-arrestin Antibody (NB100-2385) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2385AF405
Alexa Fluor 488 Labeled NB100-2385AF488
Alexa Fluor 647 Labeled NB100-2385AF647
Alexa Fluor 700 Labeled NB100-2385AF700
Biotin Labeled NB100-2385B
DyLight 350 Labeled NB100-2385UV
DyLight 405 Labeled NB100-2385V
DyLight 488 Labeled NB100-2385G
DyLight 550 Labeled NB100-2385R
DyLight 650 Labeled NB100-2385C
DyLight 680 Labeled NB100-2385FR
DyLight 755 Labeled NB100-2385IR
HRP Labeled NB100-2385H

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Blogs on S-arrestin.

"Freeze!" - Arrestin Antibodies Used in New Serotonin Syndrome Study
The beta-arrestin family regulate receptor binding of G-proteins, a group of seven transmembrane receptor proteins which includes the adrenergic, dopamine and serotonin receptors. Recently, arrestin antibodies were used in a study into Serotonin Syndr...  Read full blog post.

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Bioinformatics

Gene Symbol SAG