S-arrestin Antibody (PDS-1)

Western Blot: S-arrestin Antibody (PDS-1) [NB100-2385] - Western blot analysis of Visual Arrestin expression in human retina tissue extract.

Immunohistochemistry-Paraffin: S-arrestin Antibody (PDS-1) [NB100-2385] - S-arrestin was detected in immersion fixed paraffin-embedded sections of human prostate cancer using Mouse Anti-Human S-arrestin (PDS-1) Monoclonal Antibody (Catalog # NB100-2385) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm.

• Reactivity: Hu, Po, Bv
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: PDS-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.77 mg/ml

S-arrestin Antibody (PDS-1) Summary

• Immunogen: Porcine retinal S-arrestin [Swiss-Prot# P79260]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SAG
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Frozen 1:100-1:200
  • Immunohistochemistry-Paraffin
• Application Notes: This S-arrestin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot and the immunopurification of arrestin (PMID 3549614).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 0.77 mg/ml
• Purity: Protein G purified

Alternate Names for S-arrestin Antibody (PDS-1)

• arrestin 1
• RP47,48 kDa protein
• S-antigen; retina and pineal gland (arrestin)
• S-arrestin
• visual arrestin

Background

Arrestins family contains four types of soluble cytoplasmic proteins that act as negative regulators of GPCR signaling, wherein, cognate ligand binding stimulates GPCR phosphorylation, followed by arrestin binding to phospho-GPCR leading to receptor internalization and ultimately, the desensitization of GPCR signaling. Among four different types of mammalian arrestins, the visual proteins namely arrestin 1 (S-arrestin, S-antigen or SAG) and arrestin 4 (X-arrestin) are localized to retinal rods and cones, respectively, whereas the non-visual proteins arrestin 2 (beta-arrestin 1) and arrestin 3 (beta-arrestin 2) are ubiquitously expressed. S-arrestin is rod specific and constitutes major proteins of retinal rod outer segments and associate with photoactivated/phosphorylated rhodopsin, thereby preventing transducin-mediated phosphodiesterase activation. Besides retinal rod photoreceptor cell outer segment, S-arrestin is also expressed in the pineal gland where its function is largely unknown. Arrestin knockout mice develop photoreceptor cell degeneration because of continuous phototransduction upregulation, as a consequence of defective rhodopsin/opsin shut-off mechanism, and in human subjects, S-arrestin mutations have been linked to Oguchi disease and retinitis pigmentosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S-arrestin Antibody (NB100-2385)(1)

Publication using NB100-2385

• Banga, JP et al. Immunoaffinity purification of S-antigen using monoclonal antibodies to different antigenic sites. Invest Ophthalmol Vis Sci;28(3):604-7. 1987 Mar. [PMID: 3549614]

Bioinformatics

• Gene Symbol: SAG