RXR gamma/NR2B3 Antibody

Western Blot: RXR gamma/NR2B3 Antibody [NB100-1468] - (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RXR gamma/NR2B3 Antibody Summary

• Immunogen: Peptide with sequence RQRSRERAESEAEC corresponding to internal region according to NP_008848.1.
• Specificity: This antibody is expected to recognise an epitope corresponding to aa 213-226 of human RXR gamma protein (hinge region) and does not cross-react with either RXR alpha or beta.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RXRG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1-3 ug/ml
• Application Notes: WB: Approx. 65 kDa band observed in HeLa lysates (calculated MW of 50.9 kDa band according to NP_008848.1).Primary incubation was 1 hour. Some bands of unknown identity were also consistently observed around 37 kDa. These bands were successfully blocked by incubation with the immunising peptide.
• Control:

  RXR gamma/NR2B3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RXR gamma/NR2B3 Antibody

• NR2B3
• NR2B3retinoid X receptor-gamma
• Nuclear receptor subfamily 2 group B member 3
• retinoic acid receptor RXR-gamma
• Retinoid X receptor gamma
• retinoid X receptor, gamma
• RXR gamma
• RXRC
• RXRG

Background

Retinoids are metabolites of vitamin A (retinal) and are believed to represent important signaling molecules during vertebrate development and tissue differentiation. Two families of retinoid receptors have been identified. Retinoic acid receptors (RARs) include RAR alpha, RAR beta and RAR gamma, each of which has a high affinity for all trans retinoic acids and belongs to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. The ligand binding domains of the RARs are highly conserved and RAR isoforms are expressed in distinct patterns through out development and in the mature organism. Members of the retinoid X receptor (RXR) family, RXR alpha, RXR beta and RXR gamma, are activated by 9 cis retinoic acid, a stereo and photoisomer of all trans RA that is expressed in vivo in both liver and kidney and may represent a widely used hormone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RXR gamma/NR2B3 Antibody (NB100-1468)(1)

Publication using NB100-1468

• Samad TA, Krezel W, Chambon P, Borrelli E. Regulation of dopaminergic pathways by retinoids: activation of the D2 receptor promoter by members of the retinoic acid receptor-retinoid X receptor family. Proc Natl Acad Sci U S A 1997-12-23 [PMID: 9405615]

Bioinformatics

• Gene Symbol: RXRG
• Entrez:
  • 6258(Human)
• Uniprot:
  • NP_008848.(Human)