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RXR gamma/NR2B3 Overexpression Lysate

Images

 
Western Blot: Retinoid X Receptor gamma Overexpression Lysate (Adult Normal) [NBL1-15643] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Retinoid X Receptor ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RXR gamma/NR2B3 Overexpression Lysate Summary

Description
Transient overexpression lysate of retinoid X receptor, gamma (RXRG), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC204093 and based on accession number NM_006917. The protein contains a C-MYC/DDK Tag.
Gene
RXRG

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
50.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RXR gamma/NR2B3 Overexpression Lysate

  • NR2B3
  • NR2B3retinoid X receptor-gamma
  • Nuclear receptor subfamily 2 group B member 3
  • retinoic acid receptor RXR-gamma
  • Retinoid X receptor gamma
  • retinoid X receptor, gamma
  • RXR gamma
  • RXRC
  • RXRG

Background

This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the antiproliferative effects of retinoic acid (RA). This receptor forms dimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. This gene is expressed at significantly lower levels in non-small cell lung cancer cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for RXR gamma/NR2B3 Lysate (NBL1-15643) (0)

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Bioinformatics

Gene Symbol RXRG