![Western Blot: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody [NBP2-22523] - Analysis of 25 ug of mouse thyroid (lane 1), NIH-3T3 (lane 2) and A431 (lane 3) cell lysates onto an SDS polyacrylamide gel.](http://images.novusbio.com/fullsize2/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-Western-Blot-NBP2-22523-img0006.jpg "Western Blot: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody [NBP2-22523] - Analysis of 25 ug of mouse thyroid (lane 1), NIH-3T3 (lane 2) and A431 (lane 3) cell lysates onto an SDS polyacrylamide gel.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic Peptide: S(455) R A V C G E D D S S E A S S(469) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | THRA |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Detects an approx. 58 kDa representing Tra2 in mouse tissue. This antibody also detects a ~43 kDa protein which is believed to be non-specific. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP2-22523 | Applications | Species |
|---|---|---|
| Burda JE, O'Shea TM, Ao Y et al. Divergent transcriptional regulation of astrocyte reactivity across disorders Nature 2022-05-25 [PMID: 35614216] (IHC-Fr, Mouse) | IHC-Fr | Mouse |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody [NBP2-22523] - Analysis of Thyroid Hormone Receptor alpha-2 (green) showing staining in the nucleus and cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize2/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22523-img0003.jpg "Immunocytochemistry/Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody [NBP2-22523] - Analysis of Thyroid Hormone Receptor alpha-2 (green) showing staining in the nucleus and cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).")
![Immunohistochemistry-Paraffin: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody [NBP2-22523] - Analysis showing positive staining in the cytoplasm and nucleus of Human thyroid tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize2/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-22523-img0005.jpg "Immunohistochemistry-Paraffin: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody [NBP2-22523] - Analysis showing positive staining in the cytoplasm and nucleus of Human thyroid tissue (right) compared with a negative control in the absence of primary antibody (left).")
![Immunocytochemistry/Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody [NBP2-22523] - Analysis of Thyroid Hormone Receptor alpha-2 (green) showing staining in the nucleus and cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize2/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22523-img0001.jpg "Immunocytochemistry/Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody [NBP2-22523] - Analysis of Thyroid Hormone Receptor alpha-2 (green) showing staining in the nucleus and cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).")
![Immunocytochemistry/Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody [NBP2-22523] - Analysis of Thyroid Hormone Receptor alpha-2 (green) showing staining in the nucleus and cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize2/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22523-img0002.jpg "Immunocytochemistry/Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody [NBP2-22523] - Analysis of Thyroid Hormone Receptor alpha-2 (green) showing staining in the nucleus and cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).")
![Immunohistochemistry-Paraffin: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody [NBP2-22523] - Analysis showing positive staining in the cytoplasm and nucleus of Mouse thyroid tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize2/TR-alpha-NR1A1-Thyroid-Hormone-Receptor-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-22523-img0004.jpg "Immunohistochemistry-Paraffin: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody [NBP2-22523] - Analysis showing positive staining in the cytoplasm and nucleus of Mouse thyroid tissue (right) compared with a negative control in the absence of primary antibody (left).")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")