RXR gamma/NR2B3 Antibody (PCRP-RXRG-5H4)

Immunocytochemistry/Immunofluorescence: RXR gamma/NR2B3 Antibody (PCRP-RXRG-5H4) [NBP3-13767] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using RXR gamma/NR2B3 Antibody (PCRP-RXRG-5H4) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Flow Cytometry: RXR gamma/NR2B3 Antibody (PCRP-RXRG-5H4) [NBP3-13767] - Flow cytometric analysis of PFA-fixed HeLa cells. RXR gamma/NR2B3 antibody (PCRP-RXRG-5H4) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Protein Array: RXR gamma/NR2B3 Antibody (PCRP-RXRG-5H4) [NBP3-13767] - Analysis of Protein Array containing more than 19,000 full-length human proteins using RXR gamma/NR2B3 Antibody (PCRP-RXRG-5H4).

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-RXRG-5H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RXR gamma/NR2B3 Antibody (PCRP-RXRG-5H4) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14170)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human RXR gamma/NR2B3 protein (Uniprot: P48443)
• Localization: Nucleus,Nucleoplasm,Nuclear bodies
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RXRG
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 50.87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in Mouse, Rat and Chicken.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for RXR gamma/NR2B3 Antibody (PCRP-RXRG-5H4)

• NR2B3
• NR2B3retinoid X receptor-gamma
• Nuclear receptor subfamily 2 group B member 3
• retinoic acid receptor RXR-gamma
• Retinoid X receptor gamma
• retinoid X receptor, gamma
• RXR gamma
• RXRC
• RXRG

Background

The Retinoid X Receptor gamma gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the antiproliferative effects of retinoic acid (RA). This receptor forms dimers with the retinoic acid, thyroid hormone, and vitamin D

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RXRG
• Uniprot:
  • P48443(Human)