RXR beta/NR2B2 Antibody (MOK13-17)

Product Discontinued

RXR beta/NR2B2 Antibody (MOK13-17) Summary

• Immunogen: Recombinant human retinoic X receptor beta protein.
• Localization: Nuclear
• Specificity: This is specific for the RXR beta isoform and does not cross react with either RXR alpha or gamma.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RXRB
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 5 ug/ml
• Application Notes: WB: Detects an approx. 50 kDa protein representing RXR beta from transfected Sf9 cells. IF: Staining of RXR beta in MCF-7 cells overexpressing RXR beta results in strong nuclear staining. Gel super shift experiments show disruption of vitamin D receptor (VDR)/RXR beta/VDRE (DNA) complex formation when RXR beta was preincubated with this antibody, yet experiments using THR showed a supershift of the thyroid hormone receptor (THR)/RXR beta heterodimer by this product.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 651173). Primate reactivity reported in scientific literature (PMID: 1438648). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites diluted with PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for RXR beta/NR2B2 Antibody (MOK13-17)

• DAUDI6
• H-2RIIBP
• MHC class I promoter binding protein
• NR2B2
• NR2B2MGC1831
• Nuclear receptor subfamily 2 group B member 2
• RCoR-1
• retinoic acid receptor RXR-beta
• Retinoid X receptor beta
• retinoid X receptor, beta
• RXR beta
• RXRB

Background

Retinoid X receptors (RXRs) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. The RXR subfamily consists of at least three similar genes, RXR alpha, RXR beta and RXR gamma, all of which control transcription of target genes mediated by retinoids. RXR beta controls expression of many genes that respond to hormones and vitamins, including thyroid hormone, estrogen, retinoids and vitamin D. RXR beta controls a wide array of genes because of its ability to heterodimerize with other hormone receptors including the thyroid hormone receptor (THR), vitamin D receptor (VDR) and retinoic acid receptor (RAR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RXRB
• Entrez:
  • 6257(Human)
  • 20182(Mouse)