RXR alpha/NR2B1 Antibody

Western Blot: Retinoid X Receptor alpha Antibody [NBP2-20130] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:5000.

Retinoid X Receptor alpha antibody immunoprecipitates RXRA protein in IP experiments.IP samples: MCF-7 whole cell extractA. Control with 4 ug of preimmune Rabbit IgGB. Immunoprecipitation of RXRA protein by 4 ug Retinoid X Receptor alpha antibody (NBP2-20130)7.5 % SDS-PAGEThe immunoprecipitated RXRA protein was detected by Retinoid X Receptor alpha antibody (NBP2-20130) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Rat tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Retinoid X Receptor alpha antibody (NBP2-20130) diluted at 1:500.

• Reactivity: Hu, Rt, Mu, Bv
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RXR alpha/NR2B1 Antibody Summary

• Description: Novus Biologicals Rabbit RXR alpha/NR2B1 Antibody (NBP2-20130) is a polyclonal antibody validated for use in WB and IP. Anti-RXR alpha/NR2B1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RXR alpha/NR2B1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (99%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RXRA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:10000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RXR alpha/NR2B1 Antibody

• FLJ00280
• FLJ00318
• FLJ16020
• MGC102720
• NR2B1
• NR2B1FLJ16733
• Nuclear receptor subfamily 2 group B member 1
• retinoic acid receptor RXR-alpha
• Retinoid X receptor alpha
• retinoid X receptor, alpha
• RXR alpha
• RXRA

Background

Retinoid X receptors (RXRs) and retinoic acid receptors (RARs), are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors exert their action by binding, as homodimers or heterodimers, to specific sequences in the promoters of target genes and regulating their transcription. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for RXR alpha/NR2B1 Antibody (NBP2-20130)(1)

Publication using NBP2-20130

• Gelfand CA, Sakurai R, Wang Y et al. Inhaled vitamin A is more effective than intramuscular dosing in mitigating hyperoxia-induced lung injury in a neonatal rat model of bronchopulmonary dysplasia Am. J. Physiol. Lung Cell Mol. Physiol. 2020-08-05 [PMID: 32755324] (WB)

Bioinformatics

• Gene Symbol: RXRA
• Uniprot:
  • P19793(Human)