RRBP1 Antibody - BSA Free

Western Blot: RRBP1 Antibody [NBP1-32813] - Ribosome binding protein 1 antibody validation by siRNA knock-down. Upper panel: Ribosome binding protein 1 antibody; Lower panel: Vinculin antibodyA. 30 ug HepG2 whole cell lysate/extractB. 30 ug whole cell lysate/extract of RRBP1 siRNA#1-transfected HepG2 cellsC. 30 ug whole cell lysate/extract of RRBP1 siRNA#2-transfected HepG2 cells5% SDS-PAGE. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: RRBP1 Antibody [NBP1-32813] - Analysis of methanol-fixed HeLa, using Ribosome binding protein 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-32813] - OVCAR3 xenograft, using Ribosome binding protein 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: RRBP1 Antibody [NBP1-32813] - Sample (30 ug of whole cell lysate) A:NIH-3T3 5% SDS PAGE diluted at 1:1000

Immunocytochemistry/Immunofluorescence: RRBP1 Antibody [NBP1-32813] - Paraformaldehyde-fixed HeLa, using Ribosome binding protein 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments (Red) at 1:500.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RRBP1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human RRBP1. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum membrane, Single-pass type III membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RRBP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 152 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RRBP1 Antibody - BSA Free

• 180 kDa ribosome receptor homolog
• DKFZp586A1420
• ES/130
• ES/130-related protein
• ES130
• FLJ36146
• hES
• KIAA1398
• MGC157720
• MGC157721
• ribosome binding protein 1 (dog 180kD homolog)
• ribosome binding protein 1 homolog 180kDa (dog)
• Ribosome receptor protein
• ribosome-binding protein 1
• RRp

Background

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for RRBP1 Antibody (NBP1-32813)(1)

Publication using NBP1-32813

• Ilamathi H, Benhammouda S, Lounas A et al. Contact sites between endoplasmic reticulum sheets and mitochondria regulate mitochondrial DNA replication and segregation iScience 2023-06-01 [PMID: 37534187] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: RRBP1