RPL5 Antibody

Images

 
Western Blot: RPL5 Antibody [NBP2-22285] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22285 used for WB at 0.1 ug/ml. Detection: Chemiluminescence ...read more
Immunohistochemistry: RPL5 Antibody [NBP2-22285] - Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti- RPL5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry: RPL5 Antibody [NBP2-22285] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RPL5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: RPL5 Antibody [NBP2-22285] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22285 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated RPL5, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
   

Biological Strategies

     

Order Details

RPL5 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 247 and 297 of human Ribosomal Protein L5 using the numbering given in entry NP_000960.2 (GeneID 6125).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPL5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPL5 Antibody

  • DBA6,60S ribosomal protein L5
  • MGC117339
  • ribosomal protein L5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RPL5
Uniprot