RPA70 Antibody

Western Blot: RPA70 Antibody [NBP2-20206] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:5000.

Immunohistochemistry-Paraffin: RPA70 Antibody [NBP2-20206] - Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.

Product Discontinued

RPA70 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RPA70. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Bovine (93%), Sheep (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RPA70 Antibody

• HSSB
• MST075
• MSTP075
• REPA1RF-A protein 1
• Replication factor A protein 1
• replication protein A 70 kDa DNA-binding subunit
• replication protein A1 (70kD)
• replication protein A1, 70kDa
• RF-A
• RP-A
• RPA70RP-A p70
• Single-stranded DNA-binding protein

Background

Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RPA1
• Uniprot:
  • P27694(Human)