ROR gamma(t) Plasmid

ROR gamma(t) Plasmid [NBP2-25279] - Schematic Map of the cloning vector.

Product Discontinued

ROR gamma(t) Plasmid Summary

• Immunogen: Human ROR gamma(t) Plasmid
• Specificity: he ROR gamma(t) Plasmid is a mammalian expression vector construct, pCMV/hRORgamma(t), containing coding sequence of human RAR-related orphan receptor gamma(t) (GenBank Accession: NM_001001523).
• Gene: RORC

Applications/Dilutions

• Application Notes: This product is useful for: Expression of hROR gamma(t) protein in mammalian cells. Induction of IL-17A promoter activity. Total content of 10 ug is enough for one 96-well cell transfection (0.1 ug plasmid per well). Recommended amount for other plate formats: 6-well plate (2 ug/well), 12-well plate (0.8 ug/well) and 24-well plate (0.4 ug/well).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for ROR gamma(t) Plasmid

• NR1F3
• Nuclear receptor ROR-gamma
• Nuclear receptor RZR-gamma
• Nuclear receptor subfamily 1 group F member 3
• Retinoid-related orphan receptor-gamma
• RORC
• RORG
• RZRG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

FAQs for ROR gamma(t) Plasmid (NBP2-25279). (Showing 1 - 2 of 2 FAQ).

1. Our customers has ordered the HEK IL-17A Prom / LUCPorter (tm) Stable Reporter Cell Line (NBP2-26283) that is resistant to puromycin. Because he wanted to transfect the plasmideROR gamma (t) plasmid (NBP2-25279) which has the neomycin resistance, I made a range of neomycin on HEK IL-17A Prom / LUCPorter (tm) Stable Reporter Cell line. The result is surprising because even at a concentration of 4mg/ml which is normally lethal to HEK, cells are still alive. Do these cells contain another plasmid with a neomycin resistance? These are Hek 293 or T 293, or did you have another explanation?
   • The NBP2-26283 cell line was generated from using HEK293 cells from ATCC. Our QC test also confirmed that NBP2-26283 is neomycin resistance. NBP2-25279 assays with NBP2-26283 are based on transient transfection of NBP2-25279 into NBP2-26283. Hence there is no need for neomycin.
2. Is the neoR gene driven by a functional promoter in mammalian cells? If so, which one?
   • It is SV40 promoter that drives the neomycin gene in NBP2-25279.

Bioinformatics

• Gene Symbol: RORC