ROR gamma/RORC/NR1F3 Antibody (600214) [PerCP (Peridinin-chlorophyll Protein Complex)]

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA, Calcium Ionomycin, LPS, Recombinant Human IL-6 (Catalog # 206-IL) and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human ROR gamma/RORC/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6109C) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Product Discontinued

ROR gamma/RORC/NR1F3 Antibody (600214) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

• Immunogen: E. coli-derived recombinant human ROR gamma /RORC/NR1F3
  Met1-Gln100
  Accession # P51449-2
• Specificity: Detects human and mouse ROR gamma /RORC/NR1F3 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RORC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROR gamma/RORC/NR1F3 Antibody (600214) [PerCP (Peridinin-chlorophyll Protein Complex)]

• MGC129539
• NR1F3
• Nuclear receptor ROR gamma
• Nuclear receptor ROR-gamma
• Nuclear receptor RZR gamma
• Nuclear receptor RZR-gamma
• Nuclear receptor subfamily 1 group F member 3
• RAR-Related Orphan Nuclear Receptor Variant 2
• RAR-related orphan receptor C
• RAR-related orphan receptor gamma
• retinoic acid-binding receptor gamma
• retinoid-related orphan receptor gamma
• Retinoid-related orphan receptor-gamma
• ROR gamma
• RORC
• RORG
• RORGMGC129539
• RZRG
• RZRGRZR-GAMMA
• TOR

Background

ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family. ROR gamma is a DNA binding transcription factor. ROR gamma is 518 amino acids (aa) in length. Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis. Two splice forms differing in the first 24 aa have been found for this gene; isoform 2 deletes aa 1‑21 resulting in an alteration of aa 22‑24. Over aa 1‑100 human RORC shares 96% identity with mouse ROR gamma .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROR gamma/RORC/NR1F3 Antibody (IC6109C)(1)

Publication using IC6109C

• Wongkajornsilp A, Wamanuttajinda V, Kasetsinsombat K, Duangsa-ard S, Sa-ngiamsuntorn K, Hongeng S, Maneechotesuwan K Sunitinib indirectly enhanced anti-tumor cytotoxicity of cytokine-induced killer cells and CD3(+)CD56(+) subset through the co-culturing dendritic cells. PLoS ONE, 2013-11-13;8(11):e78980. 2013-11-13 [PMID: 24232460] (Flow Cytometry, Human)

FAQs for ROR gamma/RORC/NR1F3 Antibody (IC6109C). (Showing 1 - 3 of 3 FAQ).

1. Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?
   • Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.
2. What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?
   • The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.
3. What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?
   • A sequence from Met1 - Gln100.

Bioinformatics

• Gene Symbol: RORC
• Uniprot:
  • P51449-2()