Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [Unconjugated]

Images

 
Western blot shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑1 (Catalog # 291-G1) for 20 minutes and NIH‑3T3 mouse embryonic fibroblast cell ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 20 minutes, loaded at 0.5 mg/mL. A specific ...read more
Ribosomal Protein S6 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using 5 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-Ribosomal Protein S6 (S235/S236) Antigen Affinity-purified ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human, mouse and rat Ribosomal Protein S6 S235/S236 sites
Modification
p Ser235, p Ser236
Specificity
Detects human, mouse, and rat ribosomal protein S6 when dually phosphorylated at S235 and S236.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publication using
AF3918 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [Unconjugated]

  • Phosphoprotein NP33, 40S ribosomal protein S6
  • pS6
  • Ribosomal Protein S6
  • RPS6
  • S6

Background

40S ribosomal protein S6 is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. S6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ribosomal Protein S6/RPS6 Antibody (AF3918)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Filter By Species
Human
(1)
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Bioinformatics

Gene Symbol RPS6