Roquin Antibody

Western Blot: Roquin Antibody [NB100-656] - Samples: A) Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T, 50 ug) cells. B) Whole cell lysate (1 mg/IP; 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-656 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP (B). Roquin was also immunoprecipitated using rabbit anti-Roquin antibody NB100-655 at 3 ug/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Roquin Antibody Summary

• Description: Novus Biologicals Rabbit Roquin Antibody (NB100-656) is a polyclonal antibody validated for use in WB and IP. Anti-Roquin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1075 and the C-terminus (residue 1133) of human Ring Finger and CCCH-type finder domains 1 using the numbering given in entry NP_742068.1 (GeneID 149041).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RC3H1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:10000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Roquin Antibody

• KIAA2025RP5-1198E17.5
• RING finger and C3H zinc finger protein 1
• ring finger and CCCH-type domains 1
• RING finger protein 198
• RNF198ring finger and CCCH-type zinc finger domains 1
• roquin

Background

Roquin is a member of the RING-type E3 ubiquitin ligase protein family. It was recently found to contribute to autoimmune diseases such as type 1 diabetes and lupus. Research has found that a mutation in roquin causes T-cells to attack their own tissues. Roquin stops T-cells from displaying a stimulatory receptor, ICOS, that may cause the cells to attack normal body tissues. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Roquin Antibody (NB100-656)(1)

Publication using NB100-656

• Srivastava M, Duan G, Kershaw NJ et al. Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis Nat Commun. 2015-02-20 [PMID: 25697406] (IP, Human)

Bioinformatics

• Gene Symbol: RC3H1
• Entrez:
  • 149041(Human)
• Uniprot:
  • NP_742068.1(Human)