Roquin Antibody - BSA Free

Western Blot: Roquin Antibody [NB100-655] - Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-655 used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: Roquin Antibody [NB100-655] - Detection of human Roquin on HeLa whole cell lysate using NB100-655. Roquin was also immunoprecipitated using NB100-656 at 3 ug/mg lysate.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Roquin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Roquin Antibody - BSA Free (NB100-655) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-Roquin Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 500 and 550 of human Ring Finger and CCCH-type finder domains 1 using the numbering given in entry NP_742068.1 (GeneID 149041).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RC3H1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:500-1:5000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Use in ICC/IF reported in scientific literature (PMID: 20412057).
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Roquin Antibody - BSA Free

• KIAA2025RP5-1198E17.5
• RING finger and C3H zinc finger protein 1
• ring finger and CCCH-type domains 1
• RING finger protein 198
• RNF198ring finger and CCCH-type zinc finger domains 1
• roquin

Background

Roquin is a member of the RING-type E3 ubiquitin ligase protein family. It was recently found to contribute to autoimmune diseases such as type 1 diabetes and lupus. Research has found that a mutation in roquin causes T-cells to attack their own tissues. Roquin stops T-cells from displaying a stimulatory receptor, ICOS, that may cause the cells to attack normal body tissues. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Roquin Antibody (NB100-655)(8)

Publications using NB100-655

• Ramiscal RR, Parish IA, Lee-Young RS et al. Attenuation of AMPK signaling by ROQUIN promotes T follicular helper cell formation. Elife 2015-01-01 [PMID: 26496200]
• Murakawa Y, Hinz M, Mothes J, Schuetz A. RC3H1 post-transcriptionally regulates A20 mRNA and modulates the activity of the IKK/NF-[kappa] B pathway. NATURE COMMUNICATIONS. 2015-07-14 [PMID: 26170170] (WB, Human)
• Khoronenkova S, Dianov G ATM prevents DSB formation by coordinating SSB repair and cell cycle progression. Proc Natl Acad Sci U S A, 2015-03-16;112(13):3997-4002. 2015-03-16 [PMID: 25775545]
• Kim HJ, Ji YR, Kim MO et al. The role of Roquin overexpression in the modulation of signaling during in vitro and ex vivo T-cell activation. Biochemical and Biophysical Research Communications. 2011-11-29 [PMID: 22155229]
• Mukai Y, Yoshinaga T, Yoshikawa M et al. Induction of Endoplasmic Reticulum-Endosome Fusion for Antigen Cross-Presentation Induced by Poly ([gamma]-Glutamic Acid) Nanoparticles. Journal of Immunology (Baltimore, Md: 1950). 2011-11-16 [PMID: 22095716]
• Bertossi A, Aichinger M, Sansonetti P et al. Loss of Roquin induces early death and immune deregulation but not autoimmunity. J Exp Med 2011-08-01 [PMID: 21844204]
• Yan K, Cao Q, Reilly CM et al. Histone deacetylase 9 deficiency protects against effector T cell-mediated systemic autoimmunity. [PMID: 21708950]
• Athanasopoulos V, Barker A, Yu D et al. The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs. FEBS J. 2010-05-01 [PMID: 20412057] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: RC3H1
• Entrez:
  • 149041(Human)
• Uniprot:
  • Q5TC82(Human)