Roquin Antibody

Images

 
Western Blot: Roquin Antibody [NB100-655] - Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody ...read more
Western Blot: Roquin Antibody [NB100-655] - Detection of human Roquin on HeLa whole cell lysate using NB100-655. Roquin was also immunoprecipitated using NB100-656 at 3 ug/mg lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Roquin Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 500 and 550 of human Ring Finger and CCCH-type finder domains 1 using the numbering given in entry NP_742068.1 (GeneID 149041).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RC3H1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Histo/Cytochemistry: not tested.
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB100-655 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Roquin Antibody

  • KIAA2025RP5-1198E17.5
  • RING finger and C3H zinc finger protein 1
  • ring finger and CCCH-type domains 1
  • RING finger protein 198
  • RNF198ring finger and CCCH-type zinc finger domains 1
  • roquin

Background

Roquin is a member of the RING-type E3 ubiquitin ligase protein family. It was recently found to contribute to autoimmune diseases such as type 1 diabetes and lupus. Research has found that a mutation in roquin causes T-cells to attack their own tissues. Roquin stops T-cells from displaying a stimulatory receptor, ICOS, that may cause the cells to attack normal body tissues. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Roquin Antibody (NB100-655)(6)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Roquin Products

Bioinformatics Tool for Roquin Antibody (NB100-655)

Discover related pathways, diseases and genes to Roquin Antibody (NB100-655). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Roquin Antibody (NB100-655)

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Bioinformatics

Gene Symbol RC3H1
Entrez
Uniprot