ROCK1 Antibody

Images

 
Western Blot: ROCK1 Antibody [NB100-624] - Detection of human and mouse ROCK1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

ROCK1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1300 and the C-terminus (residue 1354) of human Rho-associated, coiled-coil containing protein kinase 1 using the numbering given in Swiss-Prot entry Q13464 (GeneID 6093).
Specificity
NB100-624 is specific for human Rho Kinase Beta protein.
Predicted Species
Rat (100%), Rabbit (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ROCK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:5000-1:15000
Application Notes
Use in IHC, IHC-P and ICC/IF reported in scientific literature (PMID 24324133).
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-624 in the following applications:

Publications
Read Publications using
NB100-624 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ROCK1 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • MGC131603
  • MGC43611
  • p160 ROCK-1
  • p160ROCK
  • p160-ROCK
  • PRO0435
  • Renal carcinoma antigen NY-REN-35
  • Rho kinase
  • rho-associated protein kinase 1
  • Rho-associated, coiled-coil containing protein kinase 1
  • Rho-associated, coiled-coil-containing protein kinase 1
  • ROCK1
  • ROK beta

Background

Rho Kinase Beta, a 160 kDa small-GTP-effector protein, is a serine/threonine kinase involved in the induction of focal adhesions and stress fibers, and can play a major role in vascular smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis. ROCK1 is identified as a substrate for caspases and is cleaved by caspase 3 at a conserved DETD1113/G sequence which removes its carboxy-terminal inhibitory domain, resulting in a constitutively active 130 kDa ROCK1 kinase. Proteolytic activation of ROCK1 by caspase is critical for its role in regulating the actin cytoskeleton and Myosin Light Chain (MLC) phosphorylation. Increased phosphorylation of MLC is important for membrane blebbing, a cellular event that occurs at the onset of apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-624
Species: Hu, Mu, Rt, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for ROCK1 Antibody (NB100-624)(3)

Reviews for ROCK1 Antibody (NB100-624) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-624:
Filter by Applications
IP
(1)
WB
(1)
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Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation ROCK1 NB100-624
Enlarge
5
reviewed by:
Verified Customer
IP Mouse 04/09/2018
View

Summary

ApplicationImmunoprecipitation
Sample TestedMC3T3-E1 cell
SpeciesMouse
LotA2
Western Blot ROCK1 NB100-624
Enlarge
5
reviewed by:
Verified Customer
WB Human 08/17/2012
View

Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 Cell Lysate
SpeciesHuman
CommentsCould have used a higher dilution or less lysate. Worked Well.

Blocking

Blocking Details5% Milk in PBS-T, 20min, RT

Primary Anitbody

Dilution Ratio1:5000 in 5% Milk in PBS-T, O/N, 4C

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG-HRP
Secondary Manufacturer Cat#GE Healthcare
Secondary Concentration1:5000

Details

Detection NotesECL Prime, 10 seconds

Comments

CommentsCould have used a higher dilution or less lysate. Worked Well.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ROCK1 Antibody (NB100-624) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
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Verified Customer
04/09/2018
Application: IP
Species: Mouse

Verified Customer
08/17/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol ROCK1
Entrez
Uniprot