RNGTT Antibody

Images

 
Western Blot: RNGTT Antibody [NBP1-32711] - Sample (30 ug of whole cell lysate) A: HeLa B: Hep G2 C: MOLT4 7.5% SDS PAGE, antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.54 mg/ml

Order Details

RNGTT Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HCE. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (95%), Rat (96%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNGTT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-32711 in the following application:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.54 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RNGTT Antibody

  • CAP1AmRNA-capping enzyme
  • hCAP
  • HCE1DKFZp686J2031
  • HCEHCAP1
  • RNA guanylyltransferase and 5'-phosphatase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RNGTT Antibody (NBP1-32711) (0)

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Review for RNGTT Antibody (NBP1-32711) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-32711:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot RNGTT NBP1-32711
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reviewed by:
Anonymous
WB Human 07/01/2014
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Summary

ApplicationWestern Blot
Sample TestedCytoplasmic extract from HEK 293 cells or U2OS cells
SpeciesHuman
LotI have used different lot and they worked very similar.

Blocking

Blocking Details3% BSA in TBS, 30-45 mins at room temperature

Primary Anitbody

Dilution Ratio1: 1000, overnight at 4.C in blocking solution

Secondary Antibody

Secondary DescriptionGoat anti-rabbit HRP
Secondary Manufacturer Cat#Santacruz SC_2030
Secondary Concentration1:10, 000

Details

Detection NotesDetected after addition of Supersignal West Femto Maximum Sensitivity substrates (Thermo), exposed for for 1- minutes

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RNGTT Antibody (NBP1-32711) (0)

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Secondary Antibodies

 

Isotype Controls

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Diseases for RNGTT Antibody (NBP1-32711)

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Calculators

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
07/01/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RNGTT
Entrez
Uniprot