RNF4 Antibody - Azide and BSA Free

Western Blot: RNF4 Antibody [H00006047-A01] - Western Blot detection against Immunogen (35.35 KDa)

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RNF4 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RNF4 (NP_002929, 107 a.a. - 190 a.a.) partial recombinant protein with GST tag. YVTTHTPRNARDEGATGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI
• Specificity: RNF4 - ring finger protein 4
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: RNF4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot
• Application Notes: The quality control of this antibody is limited to WB on the immunizing protein. It has been used for ELISA. Abnova's recommended working dilutions for western analysis are as follows: 1:500 dilution for ascites 1:1000 for purified Ig 1:500

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 50 % glycerol
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RNF4 Antibody - Azide and BSA Free

• E3 ubiquitin ligase RNF4
• EC 6.3.2.-
• Protein SNURF
• RES4-26
• ring finger protein 4SNURFSLX5
• RNF4
• SLX5
• Small nuclear ring finger protein
• SNURF

Background

The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF4 Antibody (H00006047-A01)(3)

Publications using H00006047-A01

• Alghoul E, Paloni M, Takedachi A et al. Compartmentalization of the SUMO/RNF4 pathway by SLX4 drives DNA repair Molecular cell 2023-04-05 [PMID: 37059091] (WB, KD, Human)
• Brenke JK, Popowicz GM, Schorpp K et al. Targeting TRAF6 E3 ligase activity with a small molecule inhibitor combats autoimmunity. J Biol Chem 2018-06-27 [PMID: 29950522]
• Muncheberg S, Hay RT, Ip WH et al. E1B-55K mediated regulation of RNF4 STUbL promotes HAdV gene expression. J Virol 2018-04-25 [PMID: 29695423]

Bioinformatics

• Gene Symbol: RNF4
• Entrez:
  • 6047(Human)
• OMIM:
  • 602850(Human)
• Uniprot:
  • NP_002929(Human)