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RNF23 Antibody (OTI2A6) - Azide and BSA Free

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Western Blot: RNF23 Antibody (OTI2A6) - Azide and BSA Free [NBP2-73926] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF23.
Western Blot: RNF23 Antibody (OTI2A6) - Azide and BSA Free [NBP2-73926] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF23.
Western Blot: RNF23 Antibody (OTI2A6) - Azide and BSA Free [NBP2-73926] - Analysis of extracts (35ug) from 9 different cell lines by using TRIM39 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; ...read more

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB
Clone
OTI2A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73926AF350
Alexa Fluor 405
NBP2-73926AF405
Alexa Fluor 488
NBP2-73926AF488
Alexa Fluor 532
NBP2-73926AF532
Alexa Fluor 594
NBP2-73926AF594
Alexa Fluor 647
NBP2-73926AF647
Alexa Fluor 700
NBP2-73926AF700
Alexa Fluor 750
NBP2-73926AF750
Biotin
NBP2-73926B
DyLight 350
NBP2-73926UV
DyLight 405
NBP2-73926V
DyLight 488
NBP2-73926G
DyLight 550
NBP2-73926R
DyLight 594
NBP2-73926DL594
DyLight 650
NBP2-73926C
DyLight 680
NBP2-73926FR
DyLight 755
NBP2-73926IR
HRP
NBP2-73926H
Unconjugated
NBP2-46014
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46014
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RNF23 Antibody (OTI2A6) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 144-378 of human TRIM39 (NP_742013) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TRIM39
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
Theoretical MW
56.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for RNF23 Antibody (OTI2A6) - Azide and BSA Free

  • MGC32984
  • RING finger protein 23RNF23TFPtripartite motif-containing 39
  • Testis-abundant finger protein
  • TRIM39B
  • tripartite motif containing 39
  • tripartite motif-containing protein 39

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Staining of rat brain.
Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat testis tissue.
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Western Blot: Calmodulin 2 Antibody [NBP2-14871] - Sample (50 ug of whole cell lysate) A: Mouse brain 15% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: Calmodulin 2 Antibody [NBP2-14871] - DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calmodulin 2 stained by Calmodulin 2 antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
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Calmodulin 3
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Western Blot: Calmodulin 3 Antibody [NBP2-15669] - Upregulation of miRNA inhibits the expression level of target genes in HK-2 cells and CKD patients. Western Blot shows that hsa-miR-4709-3p inhibit the protein level of ITGB8 and CALM3 respectively. NC: scrambled negative control transfection;4709: hsa-miR-4709-3p transfection. Image collected and cropped by CiteAb from the following publication (https://bmcnephrol.biomedcentral.com/articles/10.1186/s12882-019-1512-x) licensed under a CC-BY licence.
Western Blot: Calmodulin 3 Antibody [NBP2-15669] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.
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Western Blot: TJAP1 Antibody [NBP2-38152] - Analysis in control (vector only transfected HEK293T lysate) and TJAP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: TJAP1 Antibody [NBP2-38152] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: TJAP1 Antibody [NBP2-38152] - Staining of human placenta shows strong cytoplasmic positivity with a granular pattern in trophoblastic cells.
Species: Hu
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Western Blot: Regucalcin Antibody [NBP1-80849] - Analysis using Anti-RGN antibody NBP1-80849 (A) shows similar pattern to independent antibody NBP1-80850 (B).
Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Western Blot: Regucalcin Antibody [NBP1-80849] - Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate. Recommended dilution: 1:250 - 1:500
Species: Hu, Mu, Rt
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NBP1-83387
Western Blot: HADHA Antibody [NBP1-83387] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-HADHA antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: HADHA Antibody [NBP1-83387] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HADHA Antibody [NBP1-83387] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
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PRRT2 Antibody
NBP2-82026
Western Blot: PRRT2 Antibody [NBP2-82026] - Analysis of PRRT2 in mouse brain tissue lysate with PRRT2 antibody at 1 ug/ml.
Immunocytochemistry/Immunofluorescence: PRRT2 Antibody [NBP2-82026] - Immunofluorescence of PRRT2 in rat brain tissue with PRRT2 antibody at 20 ug/ml.
Immunohistochemistry: PRRT2 Antibody [NBP2-82026] - Immunohistochemistry of PRRT2 in rat brain tissue with PRRT2 antibody at 2.5 ug/ml.
Species: Hu, Mu, Rt
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CPZ Overexpression
Lysate
NBL1-09456
Western Blot: CPZ Overexpression Lysate (Adult Normal) [NBL1-09456] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPZ.
Species: Hu
TRIM10 Antibody
NBP2-85982
Western Blot: TRIM10 Antibody [NBP2-85982] - Host: Rabbit. Target Name: TRIM10. Sample Type: Human Fetal Heart. Antibody Dilution: 1.0ug/ml
Western Blot: TRIM10 Antibody [NBP2-85982] - WB Suggested Anti-TRIM10 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:2500. Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that TRIM10 is expressed in HepG2
Western Blot: TRIM10 Antibody [NBP2-85982] - Host: Rabbit. Target Name: TRIM10. Sample Type: Human Fetal Kidney. Antibody Dilution: 1.0ug/ml
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Applications: WB
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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Cytosolic
Sulfotransferase 2B1/ ...
AF6174
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IL-2 [Unconjugated]
202-IL
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(DH8.3) - EGFRvIII, M ...
NBP2-50599
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EGF [Unconjugated]
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Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
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Western Blot: DBT Antibody [NBP1-89522] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
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Insulin Antibody
(E2-E3 (same as INS04 ...
NBP2-34260
Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
(5 Reviews)
(1 Publication)
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73926AF350
Alexa Fluor 405 NBP2-73926AF405
Alexa Fluor 488 NBP2-73926AF488
Alexa Fluor 532 NBP2-73926AF532
Alexa Fluor 594 NBP2-73926AF594
Alexa Fluor 647 NBP2-73926AF647
Alexa Fluor 700 NBP2-73926AF700
Alexa Fluor 750 NBP2-73926AF750
Biotin NBP2-73926B
DyLight 350 NBP2-73926UV
DyLight 405 NBP2-73926V
DyLight 488 NBP2-73926G
DyLight 550 NBP2-73926R
DyLight 594 NBP2-73926DL594
DyLight 650 NBP2-73926C
DyLight 680 NBP2-73926FR
DyLight 755 NBP2-73926IR
HRP NBP2-73926H
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol TRIM39