RNF12 Antibody - Azide and BSA Free

Western Blot: RNF12 Antibody [H00051132-B01P] - Analysis of RNF12 expression in transfected 293T cell line by RNF12 polyclonal antibody. Lane 1: RNF12 transfected lysate(68.64 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RNF12 Antibody - Azide and BSA Free Summary

• Immunogen: RNF12 (NP_057204.2, 1 a.a. - 624 a.a.) full-length human protein. MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPPPQNSDENRGGDSSDDVSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRNNGSQNSENENEPSARRSSGENVENNSQRQVENPRSESTSARPSRSERNSTEALTEVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYSDSDSEPTGSVSNRNMERAESRSGRGGSGGGSSSGSSSSSSSSSSSSSSSSSSSSPSSSSGGESSETSSDLFEGSNEGSSSSGSSGARREGRHRAPVTFDESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLAMRSFGENDALKTCSVCITEYTEGNKLRKLPCSHEYHVHCIDRWLSENSTCPICRRAVLASGNRESVV
• Specificity: Reacts with ring finger protein 12.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: RLIM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate in western blot, and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RNF12 Antibody - Azide and BSA Free

• DKFZp686N06224
• E3 ubiquitin-protein ligase RLIM
• E3 ubiquitin-protein ligase RNF12
• EC 6.3.2
• EC 6.3.2.-
• FLJ25923
• FLJ41093
• LIM domain-interacting RING finger protein
• MGC15161
• NY-REN-43
• Renal carcinoma antigen NY-REN-43
• RING finger LIM domain-binding protein
• RING finger protein 12FLJ42887
• ring finger protein, LIM domain interacting
• ring zinc finger LIM domain binding protein
• ring zinc finger protein NY-REN-43antigen
• R-LIM
• RNF12FLJ45986

Background

The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF12 Antibody (H00051132-B01P)(2)

Publications using H00051132-B01P

• Gontan C, Mira-Bontenbal H, Magaraki A et al. REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice. Nat Commun 2018-11-12 [PMID: 30420655]
• Frints SGM, Ozanturk A, Rodriguez Criado G et al. Pathogenic variants in E3 ubiquitin ligase RLIM/RNF12 lead to a syndromic X-linked intellectual disability and behavior disorder. Mol Psychiatry 2018-05-04 [PMID: 29728705]

Bioinformatics

• Gene Symbol: RLIM
• Entrez:
  • 51132(Human)
• Uniprot:
  • NP_057204.2(Human)