RLK/TXK Antibody (674801)

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human primary differentiated Th2 cells. PVDF Membrane was probed with 1 µg/mL of Human RLK/TXK Monoclonal Antibody (Catalog # MAB6520) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RLK/TXK at approximately 58 and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 674801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

RLK/TXK Antibody (674801) Summary

• Immunogen: E.coli-derived recombinant human RLK/TXK
  Trp150-Val246
  Accession # P42681
• Specificity: Detects human RLK/TXK in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TXK
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RLK/TXK Antibody (674801)

• BTKL
• BTKLEC 2.7.10.2
• EC 2.7.10
• MGC22473
• Protein-tyrosine kinase 4
• PSCTK5
• PTK4 protein tyrosine kinase 4
• PTK4
• RLK
• TKL
• TXK tyrosine kinase
• TXK
• tyrosine kinase
• tyrosine-protein kinase TXK

Background

RLK (resting lymphocyte kinase), also called TXK (T cell-expressed kinase) or PTK4, is a protein tyrosine kinase of the TEK family. It is expressed in T cells and some myeloid cells, especially in Th1 cells in inflammatory diseases such as rheumatoid arthritis and Behcet’s disease. Upon TCR stimulation, it translocates to the nucleus and induces IFN-gamma production. An alternate start site creates a resident nuclear 55 kDa form in addition to the larger (~58 kDa) form that is cytoplasmic or targeted to the plasma membrane via palmitoylation of a cysteine string motif. The region used as an immunogen is common to both forms and shares 79% and 81% amino acid identity with mouse and rat RLK, respectively.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TXK
• Uniprot:
  • P42681()