RIPK4 Antibody [Unconjugated]

Western blot shows lysates of A549 human lung carcinoma cell line, PANC-1 human pancreatic carcinoma cell line, and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse RIPK4/RIP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10002) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RIPK4/RIP4 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

RIPK4/RIP4 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse RIPK4/RIP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10002) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

RIPK4/RIP4 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Human/Mouse RIPK4/RIP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10002) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Host: Goat
• Conjugate: Unconjugated

RIPK4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human/mouse RIPK4/RIP4
  Thr218-Thr450
  Accession # P57078
• Specificity: Detects human and mouse RIPK4/RIP4 in Western blots.
• Source: N/A
• Isotype: IgG
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.3-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RIPK4 Antibody [Unconjugated]

• ANKK2
• ANKRD3
• ANKRD3MGC129993
• ankyrin repeat domain 3
• Ankyrin repeat domain-containing protein 3
• DIKMGC129992
• EC 2.7.11
• EC 2.7.11.1
• PKC-delta-interacting protein kinase
• PKK
• receptor-interacting serine/threonine-protein kinase 4
• receptor-interacting serine-threonine kinase 4
• RIP4
• RIPK4
• serine/threonine-protein kinase ANKRD3

Background

Receptor-interacting serine-threonine kinase 4 (RIPK4/PKK) is a serine/threonine protein kinase which interacts with protein kinase C-delta, but also activates NFkappaB, and interacts with the Wnt3a coreceptor LRP6 after Wnt3a stimulation. RIPK4 is required for keratinocyte differentiation and has broad expression in epithelial tissues. Down regulation of RIPK4 has been observed in squamous cell carcinomas, hepatocellular carcinoma, urothelial carcinoma and cervical carcinoma. Mutations in in the RIPK4 gene cause Popliteal pterygium syndrome, lethal type (PPS-L), CHAND syndrome and Bartsocas-Papas syndrome.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P57078(Human)