RIN1 Antibody (27G2)

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Western Blot: RIN1 Antibody (27G2) [NBP2-66642] - Western blot analysis using Rin antibody (Cat. No. NBP2-66642) on 293 cells expressing HA-tagged Rit (1) and HA-tagged Rin (2).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
27G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RIN1 Antibody (27G2) Summary

Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with full-length recombinant human Rin protein and mouse myeloma cells.
Specificity
Does not cross-react with Rit protein.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RIN1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5
Preservative
3mM Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for RIN1 Antibody (27G2)

  • Ras and Rab interactor 1
  • ras inhibitor 1
  • Ras inhibitor JC99
  • ras inhibitor RIN1
  • Ras interaction/interference protein 1

Background

Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64% amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100% conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RIN1