RIG-I Antibody (7F6) - BSA Free

Immunohistochemistry: RIG-I Antibody (7F6) [NBP3-15174] - IHC image of RIG-I antibody diluted at 1:100 and staining in paraffin-embedded human placenta tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: 7F6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RIG-I Antibody (7F6) - BSA Free Summary

• Description: Novus Biologicals Rabbit RIG-I Antibody (7F6) - BSA Free (NBP3-15174) is a recombinant monoclonal antibody validated for use in IHC and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from human RIG-I. UniProt ID (O95786).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RIGI
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RIG-I Antibody (7F6) - BSA Free

• DDX58
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• DEAD box protein 58
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
• DKFZp686N19181
• EC 3.6.4.13
• FLJ13599
• probable ATP-dependent RNA helicase DDX58
• retinoic acid inducible gene I
• Retinoic acid-inducible gene 1 protein
• Retinoic acid-inducible gene I protein
• RIG-1
• RIGI
• RIG-I
• RIG-IDKFZp434J1111
• RNA helicase RIG-I

Background

RIG-I, also known as DDX58, consists of a 106.6 kDa and a 101.4 kDa isoform and is involved in recognizing viruses and activating a response. Current research is being conducted with diseases and disorders such as hepatitis, rubella, influenza, rabies, hemorrhagic fever, lupus nephritis, and tick-borne encephalitis. The protein interacts in pathways such as NF-kappa signaling, cytosolic DNA-sensing, measles, influenza A, and mediated induction pathways with proteins such as CYLD, ZC3HAV1, DDX3X, MAVS, and WRNIP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RIGI